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1s4g

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[[Image:1s4g.jpg|left|200px]]
 
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==Somatomedin-B Domain of human plasma vitronectin.==
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The line below this paragraph, containing "STRUCTURE_1s4g", creates the "Structure Box" on the page.
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<StructureSection load='1s4g' size='340' side='right'caption='[[1s4g]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s4g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S4G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OH:HYDROXIDE+ION'>OH</scene></td></tr>
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{{STRUCTURE_1s4g| PDB=1s4g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4g OCA], [https://pdbe.org/1s4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s4g RCSB], [https://www.ebi.ac.uk/pdbsum/1s4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4g ProSAT]</span></td></tr>
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</table>
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'''Somatomedin-B Domain of human plasma vitronectin.'''
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== Function ==
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[https://www.uniprot.org/uniprot/VTNC_HUMAN VTNC_HUMAN] Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The three-dimensional structure of an N-terminal fragment comprising the first 51 amino acids from human plasma vitronectin, the somatomedin B (SMB) domain, has been determined by two-dimensional NMR approaches. An average structure was calculated, representing the overall fold from a set of 20 minimized structures. The core residues (18-41) overlay with a root mean square deviation of 2.29 +/- 0.62 A. The N- and C-terminal segments exhibit higher root mean square deviations, reflecting more flexibility in solution and/or fewer long-range NOEs for these regions. Residues 26-30 form a unique single-turn alpha-helix, the locus where plasminogen activator inhibitor type-1 (PAI-1) is bound. This structure of this helix is highly homologous with that of a recombinant SMB domain solved in a co-crystal with PAI-1 (Zhou, A., Huntington, J. A., Pannu, N. S., Carrell, R. W., and Read, R. J. (2003) Nat. Struct. Biol. 10, 541-544), although the remainder of the structure differs. Significantly, the pattern of disulfide cross-links observed in this material isolated from human plasma is altogether different from the disulfides proposed for recombinant forms. The NMR structure reveals the relative orientation of binding sites for cell surface receptors, including an integrin-binding site at residues 45-47, which was disordered and did not diffract in the co-crystal, and a site for the urokinase receptor, which overlaps with the PAI-1-binding site.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s4g_consurf.spt"</scriptWhenChecked>
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1S4G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4G OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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The solution structure of the N-terminal domain of human vitronectin: proximal sites that regulate fibrinolysis and cell migration., Mayasundari A, Whittemore NA, Serpersu EH, Peterson CB, J Biol Chem. 2004 Jul 9;279(28):29359-66. Epub 2004 Apr 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15123712 15123712]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s4g ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Mayasundari, A.]]
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[[Category: Mayasundari A]]
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[[Category: Peterson, C B.]]
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[[Category: Peterson CB]]
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[[Category: Serpersu, E H.]]
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[[Category: Serpersu EH]]
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[[Category: Whittemore, N A.]]
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[[Category: Whittemore NA]]
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[[Category: Disulfide knot]]
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[[Category: Somatomedin b domain]]
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[[Category: Vitronectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:17:01 2008''
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Current revision

Somatomedin-B Domain of human plasma vitronectin.

PDB ID 1s4g

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