1s7a

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[[Image:1s7a.gif|left|200px]]
 
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==NMR structure of the La motif of human La protein==
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The line below this paragraph, containing "STRUCTURE_1s7a", creates the "Structure Box" on the page.
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<StructureSection load='1s7a' size='340' side='right'caption='[[1s7a]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s7a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7a OCA], [https://pdbe.org/1s7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7a RCSB], [https://www.ebi.ac.uk/pdbsum/1s7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7a ProSAT]</span></td></tr>
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{{STRUCTURE_1s7a| PDB=1s7a | SCENE= }}
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</table>
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== Function ==
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'''NMR structure of the La motif of human La protein'''
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[https://www.uniprot.org/uniprot/LA_HUMAN LA_HUMAN] Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.<ref>PMID:3192525</ref> <ref>PMID:2470590</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s7/1s7a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s7a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The La protein is a conserved component of eukaryotic ribonucleoprotein complexes that binds the 3' poly(U)-rich elements of nascent RNA polymerase III (pol III) transcripts to assist folding and maturation. This specific recognition is mediated by the N-terminal domain (NTD) of La, which comprises a La motif and an RNA recognition motif (RRM). We have determined the solution structures of both domains and show that the La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices. Chemical shift mapping experiments show that these insertions are involved in RNA interactions. They further delineate a distinct surface patch on each domain-containing both basic and aromatic residues-that interacts with RNA and accounts for the cooperative binding of short oligonucleotides exhibited by the La NTD.
The La protein is a conserved component of eukaryotic ribonucleoprotein complexes that binds the 3' poly(U)-rich elements of nascent RNA polymerase III (pol III) transcripts to assist folding and maturation. This specific recognition is mediated by the N-terminal domain (NTD) of La, which comprises a La motif and an RNA recognition motif (RRM). We have determined the solution structures of both domains and show that the La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices. Chemical shift mapping experiments show that these insertions are involved in RNA interactions. They further delineate a distinct surface patch on each domain-containing both basic and aromatic residues-that interacts with RNA and accounts for the cooperative binding of short oligonucleotides exhibited by the La NTD.
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==About this Structure==
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Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein.,Alfano C, Sanfelice D, Babon J, Kelly G, Jacks A, Curry S, Conte MR Nat Struct Mol Biol. 2004 Apr;11(4):323-9. Epub 2004 Mar 7. PMID:15004549<ref>PMID:15004549</ref>
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1S7A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7A OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein., Alfano C, Sanfelice D, Babon J, Kelly G, Jacks A, Curry S, Conte MR, Nat Struct Mol Biol. 2004 Apr;11(4):323-9. Epub 2004 Mar 7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15004549 15004549]
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</div>
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<div class="pdbe-citations 1s7a" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Alfano, C.]]
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[[Category: Alfano C]]
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[[Category: Babon, J.]]
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[[Category: Babon J]]
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[[Category: Conte, M R.]]
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[[Category: Conte MR]]
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[[Category: Curry, S.]]
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[[Category: Curry S]]
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[[Category: Jacks, A.]]
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[[Category: Jacks A]]
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[[Category: Kelly, G.]]
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[[Category: Kelly G]]
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[[Category: Sanfelice, D.]]
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[[Category: Sanfelice D]]
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[[Category: Alpha/beta]]
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[[Category: La motif]]
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[[Category: Winged helix domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:23:09 2008''
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Current revision

NMR structure of the La motif of human La protein

PDB ID 1s7a

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