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| - | [[Image:1s8k.gif|left|200px]] | |
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| - | <!-- | + | ==Solution Structure of BmKK4, A Novel Potassium Channel Blocker from Scorpion Buthus martensii Karsch, 25 structures== |
| - | The line below this paragraph, containing "STRUCTURE_1s8k", creates the "Structure Box" on the page.
| + | <StructureSection load='1s8k' size='340' side='right'caption='[[1s8k]]' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1s8k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S8K FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr> |
| - | -->
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| - | {{STRUCTURE_1s8k| PDB=1s8k | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s8k OCA], [https://pdbe.org/1s8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s8k RCSB], [https://www.ebi.ac.uk/pdbsum/1s8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s8k ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/KA171_MESMA KA171_MESMA] Blocker of potassium channels, which inhibits both the delayed rectifier and fast transient potassium current. The inhibition is reversible and voltage-independent. It causes a depolarizing shift of the steady-state activation curve of the currents, without changing their steady-state inactivation behavior.<ref>PMID:12906891</ref> |
| | + | <div style="background-color:#fffaf0;"> |
| | + | == Publication Abstract from PubMed == |
| | + | BmKK4 is a 30 amino acid peptide purified from the venom of the Chinese scorpion Buthus martensi Karsch. It has been classified as the first member of scorpion toxin subfamily alpha-KTx 17. The 3D structure of BmKK4 in solution has been determined by 2D NMR spectroscopy. This toxin adopts a common alpha/beta-motif, but shows a distinctive local conformation. The most novel feature is that the regular arrangements of the side chains of the residues involved in the beta-sheet of BmKK4 are distorted by a classic beta-bulge structure, which involves two residues (Asp18 and Arg19) in the first strand opposite a single residue (Tyr26) in the second strand. The bulge produces two main changes in the structure of the antiparallel beta-sheet: (1) It disrupts the normal alteration of the side chain direction; the side chain of Asp18 turns over to form a salt bridge with that of Arg19. (2) It accentuates the twist of the sheet, and alters the direction of the antiparallel beta-sheet. The unusual structural feature of the toxin is attributed to the shorter peptide segment (Leu15-Arg19) between the third and fourth Cys residues and two unique residues (Asp18 and Arg19) at the position preceding the fourth Cys. In addition, the lower affinity of the peptide for the Kv channel is correlated to the structural features: residue Arg19 instead of a Lys residue at the critical position for binding and the salt bridge formed between residues Arg19 and Asp18. |
| | | | |
| - | '''Solution Structure of BmKK4, A Novel Potassium Channel Blocker from Scorpion Buthus martensii Karsch, 25 structures'''
| + | Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins.,Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:15449936<ref>PMID:15449936</ref> |
| - | | + | |
| - | | + | |
| - | ==Overview==
| + | |
| - | BmKK4 is a 30 amino acid peptide purified from the venom of the Chinese scorpion Buthus martensi Karsch. It has been classified as the first member of scorpion toxin subfamily alpha-KTx 17. The 3D structure of BmKK4 in solution has been determined by 2D NMR spectroscopy. This toxin adopts a common alpha/beta-motif, but shows a distinctive local conformation. The most novel feature is that the regular arrangements of the side chains of the residues involved in the beta-sheet of BmKK4 are distorted by a classic beta-bulge structure, which involves two residues (Asp18 and Arg19) in the first strand opposite a single residue (Tyr26) in the second strand. The bulge produces two main changes in the structure of the antiparallel beta-sheet: (1) It disrupts the normal alteration of the side chain direction; the side chain of Asp18 turns over to form a salt bridge with that of Arg19. (2) It accentuates the twist of the sheet, and alters the direction of the antiparallel beta-sheet. The unusual structural feature of the toxin is attributed to the shorter peptide segment (Leu15-Arg19) between the third and fourth Cys residues and two unique residues (Asp18 and Arg19) at the position preceding the fourth Cys. In addition, the lower affinity of the peptide for the Kv channel is correlated to the structural features: residue Arg19 instead of a Lys residue at the critical position for binding and the salt bridge formed between residues Arg19 and Asp18.
| + | |
| | | | |
| - | ==About this Structure==
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | 1S8K is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mesobuthus_martensii Mesobuthus martensii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8K OCA].
| + | </div> |
| | + | <div class="pdbe-citations 1s8k" style="background-color:#fffaf0;"></div> |
| | | | |
| - | ==Reference== | + | ==See Also== |
| - | Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins., Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H, Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15449936 15449936]
| + | *[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]] |
| | + | == References == |
| | + | <references/> |
| | + | __TOC__ |
| | + | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Mesobuthus martensii]] | | [[Category: Mesobuthus martensii]] |
| - | [[Category: Single protein]]
| + | [[Category: Cao C]] |
| - | [[Category: Cao, C.]] | + | [[Category: Chen X]] |
| - | [[Category: Chen, X.]] | + | [[Category: Hu G]] |
| - | [[Category: Hu, G.]] | + | [[Category: Li M]] |
| - | [[Category: Li, M.]] | + | [[Category: Wang Y]] |
| - | [[Category: Wang, Y.]] | + | [[Category: Wu H]] |
| - | [[Category: Wu, H.]] | + | [[Category: Zhang N]] |
| - | [[Category: Zhang, N.]] | + | |
| - | [[Category: Alpha/beta scaffold]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:26:00 2008''
| + | |
| Structural highlights
Function
KA171_MESMA Blocker of potassium channels, which inhibits both the delayed rectifier and fast transient potassium current. The inhibition is reversible and voltage-independent. It causes a depolarizing shift of the steady-state activation curve of the currents, without changing their steady-state inactivation behavior.[1]
Publication Abstract from PubMed
BmKK4 is a 30 amino acid peptide purified from the venom of the Chinese scorpion Buthus martensi Karsch. It has been classified as the first member of scorpion toxin subfamily alpha-KTx 17. The 3D structure of BmKK4 in solution has been determined by 2D NMR spectroscopy. This toxin adopts a common alpha/beta-motif, but shows a distinctive local conformation. The most novel feature is that the regular arrangements of the side chains of the residues involved in the beta-sheet of BmKK4 are distorted by a classic beta-bulge structure, which involves two residues (Asp18 and Arg19) in the first strand opposite a single residue (Tyr26) in the second strand. The bulge produces two main changes in the structure of the antiparallel beta-sheet: (1) It disrupts the normal alteration of the side chain direction; the side chain of Asp18 turns over to form a salt bridge with that of Arg19. (2) It accentuates the twist of the sheet, and alters the direction of the antiparallel beta-sheet. The unusual structural feature of the toxin is attributed to the shorter peptide segment (Leu15-Arg19) between the third and fourth Cys residues and two unique residues (Asp18 and Arg19) at the position preceding the fourth Cys. In addition, the lower affinity of the peptide for the Kv channel is correlated to the structural features: residue Arg19 instead of a Lys residue at the critical position for binding and the salt bridge formed between residues Arg19 and Asp18.
Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins.,Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:15449936[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li MH, Zhang NX, Chen XQ, Wu G, Wu HM, Hu GY. BmKK4, a novel toxin from the venom of Asian scorpion Buthus martensi Karsch, inhibits potassium currents in rat hippocampal neurons in vitro. Toxicon. 2003 Aug;42(2):199-205. PMID:12906891
- ↑ Zhang N, Chen X, Li M, Cao C, Wang Y, Wu G, Hu G, Wu H. Solution structure of BmKK4, the first member of subfamily alpha-KTx 17 of scorpion toxins. Biochemistry. 2004 Oct 5;43(39):12469-76. PMID:15449936 doi:http://dx.doi.org/10.1021/bi0490643
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