We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

1sco

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:30, 1 May 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1sco.gif|left|200px]]
 
-
<!--
+
==SCORPION TOXIN (OSK1 TOXIN) WITH HIGH AFFINITY FOR SMALL CONDUCTANCE CA(2+)-ACTIVATED K+ CHANNEL IN NEUROBLASTOMA-X-GLUOMA NG 108-15 HYBRID CELLS, NMR, 30 STRUCTURES==
-
The line below this paragraph, containing "STRUCTURE_1sco", creates the "Structure Box" on the page.
+
<StructureSection load='1sco' size='340' side='right'caption='[[1sco]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1sco]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Orthochirus_scrobiculosus Orthochirus scrobiculosus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCO FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sco OCA], [https://pdbe.org/1sco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sco RCSB], [https://www.ebi.ac.uk/pdbsum/1sco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sco ProSAT]</span></td></tr>
-
{{STRUCTURE_1sco| PDB=1sco | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KAX37_ORTSC KAX37_ORTSC] Blocks voltage-gated potassium channels Kv1.1/KCNA1 (IC(50)=0.6nM), Kv1.2/KCNA2 (IC(50)=5.4nM), Kv1.3/KCNA3 (IC(50)=0.014nM) potently, and moderately block intermediate conductance calcium-activated potassium channels KCa3.1/KCNN4 (IC(50)=225nM). At high concentration (2 mM), is also active on Kv1.6/KCNA6 and Shaker IR.[REFERENCE:1]<ref>PMID:15588251</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sc/1sco_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sco ConSurf].
 +
<div style="clear:both"></div>
-
'''SCORPION TOXIN (OSK1 TOXIN) WITH HIGH AFFINITY FOR SMALL CONDUCTANCE CA(2+)-ACTIVATED K+ CHANNEL IN NEUROBLASTOMA-X-GLUOMA NG 108-15 HYBRID CELLS, NMR, 30 STRUCTURES'''
+
==See Also==
-
 
+
*[[Potassium channel toxin 3D structures|Potassium channel toxin 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
A 600 MHz 1H NMR study of toxin OSK1, blocker of small-conductance Ca2+-activated K+ channels, is presented. The unambiguous sequential assignment of all the protons of the toxin was obtained using TOCSY, DQF-COSY, and NOESY experiments at pH 3.0 (10, 30, and 45 degrees C) in aqueous solution. 3J(N alpha), 3J(alphabeta) vicinal spin coupling constants were determined in high-resolution spectra. The cross-peak volumes in NOESY spectra and the coupling constants were used to define the local structure of the protein by the program HABAS and to generate torsion angle and interproton distance constraints for the program DIANA. Hydrogen-deuterium exchange rates of amide protons showed possible locations of hydrogen bonds. The hydrogen bond acceptors and disulfide bridges between residues 8-28, 14-33, and 18-35 were determined when analyzing distance distribution in preliminary DIANA structures. All constraints were used to obtain a set of 30 structures by DIANA. The resulting rms deviations over 30 structures are 1.30 A for the heavy atoms and 0.42 A for the backbone heavy atoms. The structures were refined by constrained energy minimization using the SYBYL program. Their analysis indicated the existence of the alpha-helix (residues 10-21) slightly distorted at the Cys14 residue, two main strands of the antiparallel beta-sheet (24-29, 32-38), and the extended fragment (2-6). The motif is stabilized by the disulfide bridges in the way, common to all known scorpion toxins. Using the fine spatial toxin structure, alignment of the homologues, mutagenesis analysis, and comparison of scorpion toxin family functions, we delineate some differences significant for the toxin specificity.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
1SCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Orthochirus_scrobiculosus Orthochirus scrobiculosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCO OCA].
+
-
 
+
-
==Reference==
+
-
Three-dimensional structure of toxin OSK1 from Orthochirus scrobiculosus scorpion venom., Jaravine VA, Nolde DE, Reibarkh MJ, Korolkova YV, Kozlov SA, Pluzhnikov KA, Grishin EV, Arseniev AS, Biochemistry. 1997 Feb 11;36(6):1223-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9063870 9063870]
+
[[Category: Orthochirus scrobiculosus]]
[[Category: Orthochirus scrobiculosus]]
-
[[Category: Single protein]]
+
[[Category: Arseniev AS]]
-
[[Category: Arseniev, A S.]]
+
[[Category: Grishin EV]]
-
[[Category: Grishin, E V.]]
+
[[Category: Jaravine VA]]
-
[[Category: Jaravine, V A.]]
+
[[Category: Nolde DE]]
-
[[Category: Nolde, D E.]]
+
[[Category: Pluzhnikov KA]]
-
[[Category: Pluzhnikov, K A.]]
+
-
[[Category: Potassium channel inhibitor]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:33:03 2008''
+

Current revision

SCORPION TOXIN (OSK1 TOXIN) WITH HIGH AFFINITY FOR SMALL CONDUCTANCE CA(2+)-ACTIVATED K+ CHANNEL IN NEUROBLASTOMA-X-GLUOMA NG 108-15 HYBRID CELLS, NMR, 30 STRUCTURES

PDB ID 1sco

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sco"
Personal tools