1shn

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[[Image:1shn.gif|left|200px]]
 
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==Crystal structure of shrimp alkaline phosphatase with phosphate bound==
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The line below this paragraph, containing "STRUCTURE_1shn", creates the "Structure Box" on the page.
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<StructureSection load='1shn' size='340' side='right'caption='[[1shn]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1shn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pandalus_borealis Pandalus borealis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SHN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1shn| PDB=1shn | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1shn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1shn OCA], [https://pdbe.org/1shn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1shn RCSB], [https://www.ebi.ac.uk/pdbsum/1shn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1shn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9BHT8_PANBO Q9BHT8_PANBO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/1shn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1shn ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of shrimp alkaline phosphatase with phosphate bound'''
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==See Also==
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*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Alkaline phosphatases (APs) are homodimeric metalloenzymes that catalyze the hydrolysis and transphosphorylation of phosphate monoesters. Each monomer contains a metal-binding triad that for optimal activity is usually occupied by two zinc ions and one magnesium ion. The recently determined crystal structure of cold-active shrimp alkaline phosphatase (SAP) was, however, fully occupied by zinc ions. This paper describes a metal-exchange experiment in which the zinc ion in one binding site (referred to as the M3 site) is replaced by magnesium. Crystal structures revealed a concomitant structural change: the metal exchange causes movement of a ligating histidine into a conformation in which it does not coordinate to the metal ion. The M3 site is relevant to catalysis: its occupation by magnesium is postulated to favour catalysis and it has been suggested to be a regulatory site for other APs. Further crystallographic studies show that ligand binding can induce a conformational change of an active-site arginine from a 'non-docked' (non-interacting) to a 'docked' conformation (interacting with the ligand). The first conformation has only been observed in SAP, while the latter is common in available AP structures. The observation that the arginine does not always bind the substrate may explain the increased catalytic efficiency that is generally observed for cold-active enzymes.
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[[Category: Large Structures]]
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==About this Structure==
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1SHN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pandalus_borealis Pandalus borealis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHN OCA].
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==Reference==
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Ligand-binding and metal-exchange crystallographic studies on shrimp alkaline phosphatase., de Backer MM, McSweeney S, Lindley PF, Hough E, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1555-61. Epub 2004, Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15333925 15333925]
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[[Category: Alkaline phosphatase]]
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[[Category: Pandalus borealis]]
[[Category: Pandalus borealis]]
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[[Category: Single protein]]
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[[Category: Hough E]]
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[[Category: Backer, M M.E de.]]
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[[Category: Lindley PF]]
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[[Category: Hough, E.]]
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[[Category: McSweeney S]]
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[[Category: Lindley, P F.]]
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[[Category: De Backer MME]]
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[[Category: McSweeney, S.]]
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[[Category: Alkaline phosphatase]]
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[[Category: Beta sheet]]
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[[Category: Cold-active]]
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[[Category: Hydrolase]]
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[[Category: Metal triad]]
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[[Category: Phosphate]]
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[[Category: Phosphomonoester]]
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[[Category: Shrimp]]
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[[Category: Zinc]]
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[[Category: Zinc triad]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:42:54 2008''
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Current revision

Crystal structure of shrimp alkaline phosphatase with phosphate bound

PDB ID 1shn

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