1sig

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[[Image:1sig.jpg|left|200px]]
 
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==CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE==
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The line below this paragraph, containing "STRUCTURE_1sig", creates the "Structure Box" on the page.
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<StructureSection load='1sig' size='340' side='right'caption='[[1sig]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1sig]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SIG FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sig OCA], [https://pdbe.org/1sig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sig RCSB], [https://www.ebi.ac.uk/pdbsum/1sig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sig ProSAT]</span></td></tr>
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{{STRUCTURE_1sig| PDB=1sig | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOD_ECOLI RPOD_ECOLI] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/si/1sig_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sig ConSurf].
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<div style="clear:both"></div>
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'''CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE'''
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==See Also==
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*[[Sigma factor 3D structures|Sigma factor 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Darst SA]]
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1SIG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIG OCA].
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[[Category: Malhotra A]]
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[[Category: Severinova E]]
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==Reference==
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Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase., Malhotra A, Severinova E, Darst SA, Cell. 1996 Oct 4;87(1):127-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8858155 8858155]
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[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Darst, S A.]]
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[[Category: Malhotra, A.]]
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[[Category: Severinova, E.]]
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[[Category: Rna polymerase sigma factor]]
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[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:44:39 2008''
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Current revision

CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE

PDB ID 1sig

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