1sky

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:31, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1sky.gif|left|200px]]
 
-
<!--
+
==CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3==
-
The line below this paragraph, containing "STRUCTURE_1sky", creates the "Structure Box" on the page.
+
<StructureSection load='1sky' size='340' side='right'caption='[[1sky]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1sky]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._PS3 Bacillus sp. PS3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKY FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
{{STRUCTURE_1sky| PDB=1sky | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sky OCA], [https://pdbe.org/1sky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sky RCSB], [https://www.ebi.ac.uk/pdbsum/1sky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sky ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ATPA_BACP3 ATPA_BACP3] Produces ATP from ADP in the presence of a proton gradient across the membrane. The alpha chain is a regulatory subunit.[HAMAP-Rule:MF_01346]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1sky_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sky ConSurf].
 +
<div style="clear:both"></div>
-
'''CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3'''
+
==See Also==
-
 
+
*[[ATPase 3D structures|ATPase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
BACKGROUND: F1-ATPase, an oligomeric assembly with subunit stoichiometry alpha 3 beta 3 gamma delta epsilon, is the catalytic component of the ATP synthase complex, which plays a central role in energy transduction in bacteria, chloroplasts and mitochondria. The crystal structure of bovine mitochondrial F1-ATPase displays a marked asymmetry in the conformation and nucleotide content of the catalytic beta subunits. The alpha 3 beta 3 subcomplex of F1-ATPase has been assembled from subunits of the moderately thermophilic Bacillus PS3 made in Escherichia coli, and the subcomplex is active but does not show the catalytic cooperativity of intact F1-ATPase. The structure of this subcomplex should provide new information on the conformational variability of F1-ATPase and may provide insights into the unusual catalytic mechanism employed by this enzyme. RESULTS: The crystal structure of the nucleotide-free bacterial alpha 3 beta 3 subcomplex of F1-ATPase, determined at 3.2 A resolution, shows that the oligomer has exact threefold symmetry. The bacterial beta subunits adopt a conformation essentially identical to that of the nucleotide-free beta subunit in mitochondrial F1-ATPase; the alpha subunits have similar conformations in both structures. CONCLUSIONS: The structures of the bacterial F1-ATPase alpha and beta subunits are very similar to their counterparts in the mitochondrial enzyme, suggesting a common catalytic mechanism. The study presented here allows an analysis of the different conformations adopted by the alpha and beta subunits and may ultimately further our understanding of this mechanism.
+
[[Category: Bacillus sp. PS3]]
-
 
+
[[Category: Large Structures]]
-
==About this Structure==
+
[[Category: Abrahams JP]]
-
1SKY is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_sp._ps3 Bacillus sp. ps3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKY OCA].
+
[[Category: Kagawa Y]]
-
 
+
[[Category: Kambara M]]
-
==Reference==
+
[[Category: Leslie AGW]]
-
The crystal structure of the nucleotide-free alpha 3 beta 3 subcomplex of F1-ATPase from the thermophilic Bacillus PS3 is a symmetric trimer., Shirakihara Y, Leslie AG, Abrahams JP, Walker JE, Ueda T, Sekimoto Y, Kambara M, Saika K, Kagawa Y, Yoshida M, Structure. 1997 Jun 15;5(6):825-36. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9261073 9261073]
+
[[Category: Saika K]]
-
[[Category: Bacillus sp. ps3]]
+
[[Category: Sekimoto Y]]
-
[[Category: Protein complex]]
+
[[Category: Shirakihara Y]]
-
[[Category: Abrahams, J P.]]
+
[[Category: Ueda T]]
-
[[Category: Kagawa, Y.]]
+
[[Category: Walker JE]]
-
[[Category: Kambara, M.]]
+
[[Category: Yoshida M]]
-
[[Category: Leslie, A G.W.]]
+
-
[[Category: Saika, K.]]
+
-
[[Category: Sekimoto, Y.]]
+
-
[[Category: Shirakihara, Y.]]
+
-
[[Category: Ueda, T.]]
+
-
[[Category: Walker, J E.]]
+
-
[[Category: Yoshida, M.]]
+
-
[[Category: Alpha3beta3 subcomplex of f1-atpase]]
+
-
[[Category: Atp synthase]]
+
-
[[Category: F1-atpase]]
+
-
[[Category: F1fo atp synthase]]
+
-
[[Category: Hydrolase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:49:58 2008''
+

Current revision

CRYSTAL STRUCTURE OF THE NUCLEOTIDE FREE ALPHA3BETA3 SUB-COMPLEX OF F1-ATPASE FROM THE THERMOPHILIC BACILLUS PS3

PDB ID 1sky

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1sky"
Personal tools