1smt

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[[Image:1smt.gif|left|200px]]
 
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==SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942==
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The line below this paragraph, containing "STRUCTURE_1smt", creates the "Structure Box" on the page.
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<StructureSection load='1smt' size='340' side='right'caption='[[1smt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1smt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SMT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1smt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1smt OCA], [https://pdbe.org/1smt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1smt RCSB], [https://www.ebi.ac.uk/pdbsum/1smt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1smt ProSAT]</span></td></tr>
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{{STRUCTURE_1smt| PDB=1smt | SCENE= }}
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</table>
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== Function ==
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'''SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942'''
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[https://www.uniprot.org/uniprot/SMTB_SYNE7 SMTB_SYNE7] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.<ref>PMID:12146943</ref> <ref>PMID:12407207</ref> <ref>PMID:8871549</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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SmtB from Synechococcus PCC7942 is a trans-acting dimeric repressor that is required for Zn(2+)-responsive expression of the metallothionein SmtA. The structure of SmtB was solved using multiple isomorphous replacement techniques and refined at 2.2 A resolution by simulated annealing to an R-factor of 0.218. SmtB displays the classical helix-turn-helix motif found in many DNA-binding proteins. It has an alpha + beta topology, and the arrangement of the three core helices and the beta hairpin is similar to the HNF-3/fork head, CAP and diphtheria toxin repressor proteins. Although there is no zinc in the crystal structure, analysis of a mercuric acetate derivative suggests a total of four Zn2+ binding sites in the dimer. Two of these putative sites are at the opposite ends of the dimer, while the other two are at the dimer interface and are formed by residues contributed from each monomer. The structure of the dimer is such that simultaneous binding for both recognition helices to DNA would require either a bend in the DNA helix or a conformational change in the dimer. The structure of Synechococcus SmtB is the first in this family of metal-binding DNA repressors.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sm/1smt_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1SMT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMT OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1smt ConSurf].
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Crystal structure of the cyanobacterial metallothionein repressor SmtB: a model for metalloregulatory proteins., Cook WJ, Kar SR, Taylor KB, Hall LM, J Mol Biol. 1998 Jan 16;275(2):337-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9466913 9466913]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Synechococcus sp.]]
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<references/>
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[[Category: Cook, W J.]]
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__TOC__
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[[Category: Hall, L M.]]
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</StructureSection>
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[[Category: Dna repressor]]
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[[Category: Large Structures]]
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[[Category: Metalloregulation]]
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[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
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[[Category: Zinc]]
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[[Category: Cook WJ]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:54:03 2008''
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[[Category: Hall LM]]

Current revision

SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942

PDB ID 1smt

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