1svx

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[[Image:1svx.gif|left|200px]]
 
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==Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein==
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The line below this paragraph, containing "STRUCTURE_1svx", creates the "Structure Box" on the page.
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<StructureSection load='1svx' size='340' side='right'caption='[[1svx]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1svx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SVX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1svx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1svx OCA], [https://pdbe.org/1svx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1svx RCSB], [https://www.ebi.ac.uk/pdbsum/1svx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1svx ProSAT]</span></td></tr>
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{{STRUCTURE_1svx| PDB=1svx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sv/1svx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1svx ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein'''
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==See Also==
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*[[Ankyrin 3D structures|Ankyrin 3D structures]]
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*[[Maltose-binding protein 3D structures|Maltose-binding protein 3D structures]]
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==Overview==
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__TOC__
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We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding. Using a consensus design strategy, we generated combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces. Libraries of two and three repeats, flanked by 'capping repeats,' were used in ribosome-display selections against maltose binding protein (MBP) and two eukaryotic kinases. We rapidly enriched target-specific binders with affinities in the low nanomolar range and determined the crystal structure of one of the selected AR proteins in complex with MBP at 2.3 A resolution. The interaction relies on the randomized positions of the designed AR protein and is comparable to natural, heterodimeric protein-protein interactions. Thus, our AR protein libraries are valuable sources for binding molecules and, because of the very favorable biophysical properties of the designed AR proteins, an attractive alternative to antibody libraries.
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</StructureSection>
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==About this Structure==
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1SVX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVX OCA].
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==Reference==
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High-affinity binders selected from designed ankyrin repeat protein libraries., Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter MG, Pluckthun A, Nat Biotechnol. 2004 May;22(5):575-82. Epub 2004 Apr 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15097997 15097997]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Amstutz, P.]]
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[[Category: Amstutz P]]
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[[Category: Binz, H K.]]
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[[Category: Binz HK]]
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[[Category: Briand, C.]]
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[[Category: Briand C]]
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[[Category: Forrer, P.]]
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[[Category: Forrer P]]
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[[Category: Gruetter, M G.]]
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[[Category: Gruetter MG]]
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[[Category: Kohl, A.]]
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[[Category: Kohl A]]
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[[Category: Plueckthun, A.]]
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[[Category: Plueckthun A]]
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[[Category: Stumpp, M T.]]
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[[Category: Stumpp MT]]
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[[Category: Ankyrin repeat protein]]
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[[Category: Artificial complex]]
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[[Category: Protein design]]
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[[Category: Selected binder]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:12:01 2008''
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Current revision

Crystal structure of a designed selected Ankyrin Repeat protein in complex with the Maltose Binding Protein

PDB ID 1svx

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