1swi

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[[Image:1swi.gif|left|200px]]
 
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==GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE==
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The line below this paragraph, containing "STRUCTURE_1swi", creates the "Structure Box" on the page.
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<StructureSection load='1swi' size='340' side='right'caption='[[1swi]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1swi]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SWI FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNZ:BENZENE'>BNZ</scene></td></tr>
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{{STRUCTURE_1swi| PDB=1swi | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1swi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swi OCA], [https://pdbe.org/1swi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1swi RCSB], [https://www.ebi.ac.uk/pdbsum/1swi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1swi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GCN4_YEAST GCN4_YEAST] Is a transcription factor that is responsible for the activation of more than 30 genes required for amino acid or for purine biosynthesis in response to amino acid or purine starvation. Binds and recognize the DNA sequence: 5'-TGA[CG]TCA-3'.
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'''GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE'''
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==See Also==
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*[[Gcn4 3D Structures|Gcn4 3D Structures]]
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*[[Gnc4 3D Structures|Gnc4 3D Structures]]
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==Overview==
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__TOC__
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Controversy remains about the role of core side-chain packing in specifying protein structure. To investigate the influence of core packing on the oligomeric structure of a coiled coil, we engineered a GCN4 leucine zipper mutant that switches from two to three strands upon binding the hydrophobic ligands cyclohexane and benzene. In solution these ligands increased the apparent thermal stability and the oligomerization order of the mutant leucine zipper. The crystal structure of the peptide-benzene complex shows a single benzene molecule bound at the engineered site in the core of the trimer. These results indicate that coiled coils are well-suited to function as molecular switches and emphasize that core packing is an important determinant of oligomerization specificity.
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</StructureSection>
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[[Category: Large Structures]]
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==About this Structure==
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1SWI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWI OCA].
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==Reference==
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An engineered allosteric switch in leucine-zipper oligomerization., Gonzalez L Jr, Plecs JJ, Alber T, Nat Struct Biol. 1996 Jun;3(6):510-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8646536 8646536]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Alber T]]
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[[Category: Alber, T.]]
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[[Category: Gonzalez L]]
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[[Category: Gonzalez, L.]]
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[[Category: Plecs J]]
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[[Category: Plecs, J.]]
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[[Category: Coiled coil]]
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[[Category: Leucine zipper]]
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[[Category: Peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:13:12 2008''
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Current revision

GCN4-LEUCINE ZIPPER CORE MUTANT AS N16A COMPLEXED WITH BENZENE

PDB ID 1swi

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