9ilt
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 9ilt is ON HOLD until Paper Publication Authors: Xu, X., Wu, W. Description: Crystal structure of alternative complex III from Chloroflexus auranti...) |
|||
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of alternative complex III from Chloroflexus aurantiacus== | |
| + | <StructureSection load='9ilt' size='340' side='right'caption='[[9ilt]], [[Resolution|resolution]] 3.25Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9ilt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus_J-10-fl Chloroflexus aurantiacus J-10-fl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9ILT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9ILT FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ilt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ilt OCA], [https://pdbe.org/9ilt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ilt RCSB], [https://www.ebi.ac.uk/pdbsum/9ilt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ilt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A9WEV4_CHLAA A9WEV4_CHLAA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Alternative complex III (ACIII) is a multi-subunit quinol:electron acceptor oxidoreductase that couples quinol oxidation with transmembrane proton translocation in bacterial respiratory and photosynthetic electron transport chains. Four ACIII cryoelectron microscopy (cryo-EM) structures are known. However, the effects of cryo-EM versus X-ray crystallography structure determination on ACIII structure are unclear. Here, we report a 3.25 A crystal structure of photosynthetic ACIII from Chloroflexus aurantiacus (CaACIIIp), revealing eight subunits (ActA-G and I) with four iron-sulfur clusters and six c-type hemes, a menaquinol-binding site, and two proton translocation passages. Structural comparisons with the previously reported cryo-EM structures reveal slight local conformational changes in the solvent-exposed regions of ActB, ActD, ActG, and the transmembrane (TM) helix of subunit I. The regions conferring structural flexibility possess low sequence conservation across species. However, the core functional modules containing the menaquinol-binding pocket, redox centers, and proton translocation passages remain unchanged, preserving the enzyme's activity. | ||
| - | + | Crystal structure of the alternative complex III from the phototrophic bacterium Chloroflexus aurantiacus.,Wu W, Fang H, He H, Wu J, Gong Z, Li C, Pei X, Xu X Structure. 2025 Jan 2;33(1):29-38.e2. doi: 10.1016/j.str.2024.10.014. Epub 2024 , Nov 4. PMID:39500318<ref>PMID:39500318</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Wu | + | <div class="pdbe-citations 9ilt" style="background-color:#fffaf0;"></div> |
| - | [[Category: Xu | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chloroflexus aurantiacus J-10-fl]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Wu W]] | ||
| + | [[Category: Xu X]] | ||
Current revision
Crystal structure of alternative complex III from Chloroflexus aurantiacus
| |||||||||||
