9g3p
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Circularly permuted lumazine synthase 12-pentamer spherical cage== | |
| + | <StructureSection load='9g3p' size='340' side='right'caption='[[9g3p]], [[Resolution|resolution]] 2.08Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9g3p]] is a 60 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus_VF5 Aquifex aeolicus VF5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9G3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9G3P FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.08Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9g3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9g3p OCA], [https://pdbe.org/9g3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9g3p RCSB], [https://www.ebi.ac.uk/pdbsum/9g3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9g3p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/RISB_AQUAE RISB_AQUAE] Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.<ref>PMID:12603336</ref> <ref>PMID:11237620</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hollow proteinaceous particles are useful nanometric containers for delivery and catalysis. Understanding the molecular mechanisms and the geometrical theory behind the polymorphic protein assemblies provides a basis for designing ones with the desired morphology. As such, we found that a circularly permuted variant of a cage-forming enzyme, Aquifex aeolicus lumazine synthase, cpAaLS, assembles into a variety of hollow spherical and cylindrical structures in response to changes in ionic strength. Cryogenic electron microscopy revealed that these structures are composed entirely of pentameric subunits, and the dramatic cage-to-tube transformation is attributed to the moderately hindered 3-fold symmetry interaction and the imparted torsion angle of the building blocks, where both mechanisms are mediated by an alpha-helix domain that is untethered from the native position by circular permutation. Mathematical modeling suggests that the unique double- and triple-stranded helical arrangements of subunits are optimal tiling patterns, while different geometries should be possible by modulating the interaction angles of the pentagons. These structural insights into dynamic, pentamer-based protein cages and nanotubes afford guidelines for designing nanoarchitectures with customized morphology and assembly characteristics. | ||
| - | + | Dynamic Assembly of Pentamer-Based Protein Nanotubes.,Koziej L, Fatehi F, Aleksejczuk M, Byrne MJ, Heddle JG, Twarock R, Azuma Y ACS Nano. 2025 Feb 24. doi: 10.1021/acsnano.4c16192. PMID:39993171<ref>PMID:39993171</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Azuma | + | <div class="pdbe-citations 9g3p" style="background-color:#fffaf0;"></div> |
| - | [[Category: Koziej | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aquifex aeolicus VF5]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Azuma Y]] | ||
| + | [[Category: Koziej L]] | ||
Current revision
Circularly permuted lumazine synthase 12-pentamer spherical cage
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