1wrm

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(New page: 200px<br /> <applet load="1wrm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wrm, resolution 1.50&Aring;" /> '''Crystal structure o...)
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[[Image:1wrm.gif|left|200px]]<br />
 
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<applet load="1wrm" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1wrm, resolution 1.50&Aring;" />
 
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'''Crystal structure of JSP-1'''<br />
 
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==Overview==
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==Crystal structure of JSP-1==
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Human JNK stimulatory phosphatase-1 (JSP-1) is a novel member of dual, specificity phosphatases. A C-terminus truncated JSP-1 was expressed in, Escherichia coli and was crystallized using the sitting-drop vapor, diffusion method. Thin-plate crystals obtained at 278 K belong to a, monoclinic space group, C2, with unit-cell parameters a = 84.0 A, b = 49.3, A, c = 47.3 A, and beta = 119.5 degrees , and diffract up to 1.5 A, resolution at 100 K. The structure of JSP-1 has a single compact, (alpha/beta) domain, which consists of six alpha-helices and five, beta-strands, and shows a conserved structural scaffold in regard to both, DSPs and PTPs. A cleft formed by a PTP-loop at the active site is very, shallow, and is occupied by one sulfonate compound, MES, at the bottom. In, the binary complex structure of JSP-1 with MES, the conformations of three, important segments in regard to the catalytic mechanism are not similar to, those in PTP1B. JSP-1 has no loop corresponding to the Lys120-loop of, PTP1B, and tryptophan residue corresponding to the substrate-stacking in, PTP1B is substituted by alanine residue in JSP-1.
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<StructureSection load='1wrm' size='340' side='right'caption='[[1wrm]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1wrm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WRM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wrm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wrm OCA], [https://pdbe.org/1wrm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wrm RCSB], [https://www.ebi.ac.uk/pdbsum/1wrm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wrm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DUS22_HUMAN DUS22_HUMAN] Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) (By similarity).<ref>PMID:11717427</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wr/1wrm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wrm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1WRM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MES as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1WRM OCA].
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*[[Dual specificity phosphatase 3D structures|Dual specificity phosphatase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 A resolution., Yokota T, Nara Y, Kashima A, Matsubara K, Misawa S, Kato R, Sugio S, Proteins. 2007 Feb 1;66(2):272-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17068812 17068812]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein-tyrosine-phosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Kashima A]]
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[[Category: Kashima, A.]]
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[[Category: Kato R]]
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[[Category: Kato, R.]]
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[[Category: Sugio S]]
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[[Category: Sugio, S.]]
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[[Category: Yokota T]]
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[[Category: Yokota, T.]]
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[[Category: MES]]
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[[Category: dsp]]
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[[Category: jnk]]
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[[Category: phosphatase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:54:10 2007''
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Current revision

Crystal structure of JSP-1

PDB ID 1wrm

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