9cv8
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal Structure of Cytochrome P450 NysL bound to nystatin== | |
| + | <StructureSection load='9cv8' size='340' side='right'caption='[[9cv8]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9cv8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_noursei Streptomyces noursei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9CV8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9CV8 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1AZ7:(1~{S},3~{R},4~{R},7~{R},9~{R},11~{R},15~{S},16~{R},17~{R},18~{S},19~{E},21~{E},25~{E},27~{E},29~{E},31~{E},33~{R},35~{S},36~{R},37~{S})-33-[(2~{R},3~{S},4~{S},5~{S},6~{R})-4-azanyl-6-methyl-3,5-bis(oxidanyl)oxan-2-yl]oxy-15,16,18-trimethyl-1,3,4,7,9,11,17,37-octakis(oxidanyl)-13-oxidanylidene-14,39-dioxabicyclo[33.3.1]nonatriaconta-19,21,25,27,29,31-hexaene-36-carboxylic+acid'>A1AZ7</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9cv8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9cv8 OCA], [https://pdbe.org/9cv8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9cv8 RCSB], [https://www.ebi.ac.uk/pdbsum/9cv8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9cv8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q9L4X0_STRNR Q9L4X0_STRNR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | NysL, a cytochrome P450 monooxygenase from the Gram-positive bacterium Streptomyces noursei, catalyzes the C10 hydroxylation of 10-deoxynystain to nystatin A(1), a clinically important antifungal. In this study, we present the 2.0 A resolution crystal structure of NysL bound to nystatin A(1). The structure of this complex provides key insights into the structural elements that dictate the regio- and stereo- selective oxidation of large 20-44-membered macrolide substrates. The closely related AmphL operates on a similar 38-member macrolide but oxidizes C8 rather than C10. This difference requires that the substrate for AmphL penetrate further into the active site relative to NysL. The depth of substrate penetration is controlled by interactions between an area of the substrate binding pocket deemed the "back-wall" and the hemiketal ring of the macrolide substrate. | ||
| - | + | Crystal structure of cytochrome P450 NysL and the structural basis for stereo- and regio- selective oxidation of antifungal macrolides.,Murarka VC, Kim JS, Lamb DC, Kelly SL, Poulos TL, Follmer AH J Biol Chem. 2025 Jan 13:108185. doi: 10.1016/j.jbc.2025.108185. PMID:39814230<ref>PMID:39814230</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Murarka | + | <div class="pdbe-citations 9cv8" style="background-color:#fffaf0;"></div> |
| - | [[Category: Poulos | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces noursei]] | ||
| + | [[Category: Murarka VC]] | ||
| + | [[Category: Poulos TL]] | ||
Current revision
Crystal Structure of Cytochrome P450 NysL bound to nystatin
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