9go5
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM Reconstruction of Yeast ADP-Actin Filament at 2.5 A resolution.== | |
| + | <StructureSection load='9go5' size='340' side='right'caption='[[9go5]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9go5]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GO5 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9go5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9go5 OCA], [https://pdbe.org/9go5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9go5 RCSB], [https://www.ebi.ac.uk/pdbsum/9go5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9go5 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ACT_YEAST ACT_YEAST] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The core component of the actin cytoskeleton is the globular protein G-actin, which reversibly polymerizes into filaments (F-actin). Budding yeast possesses a single actin that shares 87%-89% sequence identity with vertebrate actin isoforms. Previous structural studies indicate very close overlap of main-chain backbones. Intriguingly, however, substitution of yeast ACT1 with vertebrate beta-cytoplasmic actin severely disrupts cell function and the substitution with a skeletal muscle isoform is lethal. Here we report a 2.5 A structure of budding yeast F-actin. Previously unresolved side-chain information allows us to highlight four main differences in the comparison of yeast and vertebrate ADP F-actins: a more open nucleotide binding pocket; a more solvent exposed C-terminus; a rearrangement of inter-subunit binding interactions in the vicinity of the D loop and changes in the hydrogen bonding network in the vicinity of histidine 73 (yeast actin) and methyl-histidine 73 (vertebrate actin). | ||
| - | + | Cryo-EM reconstruction of yeast ADP-actin filament at 2.5 A resolution. A comparison with vertebrate F-actin.,Stevenson SR, Tzokov SB, Lahiri I, Ayscough KR, Bullough PA Structure. 2025 Jan 2:S0969-2126(24)00543-4. doi: 10.1016/j.str.2024.12.008. PMID:39798573<ref>PMID:39798573</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9go5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae]] | ||
| + | [[Category: Ayscough KR]] | ||
| + | [[Category: Bullough PA]] | ||
| + | [[Category: Lahiri I]] | ||
| + | [[Category: Stevenson SR]] | ||
| + | [[Category: Tzokov SB]] | ||
Current revision
CryoEM Reconstruction of Yeast ADP-Actin Filament at 2.5 A resolution.
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