1t85

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:37, 14 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1t85.gif|left|200px]]
 
-
<!--
+
==Crystal Structure of the Ferrous CO-bound Cytochrome P450cam Mutant (L358P/C334A)==
-
The line below this paragraph, containing "STRUCTURE_1t85", creates the "Structure Box" on the page.
+
<StructureSection load='1t85' size='340' side='right'caption='[[1t85]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1t85]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T85 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T85 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAM:CAMPHOR'>CAM</scene>, <scene name='pdbligand=CMO:CARBON+MONOXIDE'>CMO</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-
{{STRUCTURE_1t85| PDB=1t85 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t85 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t85 OCA], [https://pdbe.org/1t85 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t85 RCSB], [https://www.ebi.ac.uk/pdbsum/1t85 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t85 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CPXA_PSEPU CPXA_PSEPU] Involved in a camphor oxidation system.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t85_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t85 ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal Structure of the Ferrous CO-bound Cytochrome P450cam Mutant (L358P/C334A)'''
+
==See Also==
-
 
+
*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The cytochrome P450cam active site is known to be perturbed by binding to its redox partner, putidaredoxin (Pdx). Pdx binding also enhances the camphor monooxygenation reaction (Nagano, S., Shimada, H., Tarumi, A., Hishiki, T., Kimata-Ariga, Y., Egawa, T., Suematsu, M., Park, S.-Y., Adachi, S., Shiro, Y., and Ishimura, Y. (2003) Biochemistry 42, 14507-14514). These effects are unique to Pdx because nonphysiological electron donors are unable to support camphor monooxygenation. The accompanying 1H NMR paper (Tosha, T., Yoshioka, S., Ishimori, K., and Morishima, I. (2004) J. Biol. Chem. 279, 42836-42843) shows that the conformation of active site residues, Thr-252 and Cys-357, and the substrate in the ferrous (Fe(II)) CO complex of the L358P mutant mimics that of the wild-type enzyme complexed to Pdx. To explore how these changes are transmitted from the Pdx-binding site to the active site, we have solved the crystal structures of the ferrous and ferrous-CO complex of wild-type and the L358P mutant. Comparison of these structures shows that the L358P mutation results in the movement of Arg-112, a residue known to be important for putidaredoxin binding, toward the heme. This change could optimize the Pdx-binding site leading to a higher affinity for Pdx. The mutation also pushes the heme toward the substrate and ligand binding pocket, which relocates the substrate to a position favorable for regio-selective hydroxylation. The camphor is held more firmly in place as indicated by a lower average temperature factor. Residues involved in the catalytically important proton shuttle system in the I helix are also altered by the mutation. Such conformational alterations and the enhanced reactivity of the mutant oxy complex with non-physiological electron donors suggest that Pdx binding optimizes the distal pocket for monooxygenation of camphor.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
1T85 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T85 OCA].
+
-
 
+
-
==Reference==
+
-
Crystal structure of the cytochrome p450cam mutant that exhibits the same spectral perturbations induced by putidaredoxin binding., Nagano S, Tosha T, Ishimori K, Morishima I, Poulos TL, J Biol Chem. 2004 Oct 8;279(41):42844-9. Epub 2004 Jul 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15269210 15269210]
+
-
[[Category: Camphor 5-monooxygenase]]
+
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]
-
[[Category: Single protein]]
+
[[Category: Ishimori K]]
-
[[Category: Ishimori, K.]]
+
[[Category: Morishima I]]
-
[[Category: Morishima, I.]]
+
[[Category: Nagano S]]
-
[[Category: Nagano, S.]]
+
[[Category: Poulos TL]]
-
[[Category: Poulos, T L.]]
+
[[Category: Tosha T]]
-
[[Category: Tosha, T.]]
+
-
[[Category: Cytochrome p450]]
+
-
[[Category: Heme enzyme]]
+
-
[[Category: Oxidoreductase]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:39:09 2008''
+

Current revision

Crystal Structure of the Ferrous CO-bound Cytochrome P450cam Mutant (L358P/C334A)

PDB ID 1t85

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1t85"
Personal tools