1x0x

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(New page: 200px<br /> <applet load="1x0x" size="450" color="white" frame="true" align="right" spinBox="true" caption="1x0x, resolution 2.75&Aring;" /> '''Co-Structure of Hom...)
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[[Image:1x0x.gif|left|200px]]<br />
 
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<applet load="1x0x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1x0x, resolution 2.75&Aring;" />
 
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'''Co-Structure of Homo Sapiens Glycerol-3-Phosphate Dehydrogenase 1 complex with NAD'''<br />
 
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==Overview==
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==Co-Structure of Homo Sapiens Glycerol-3-Phosphate Dehydrogenase 1 complex with NAD==
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Homo sapiens L-alpha-glycerol-3-phosphate dehydrogenase 1 (GPD1) catalyzes, the reversible biological conversion of dihydroxyacetone (DHAP) to, glycerol-3-phosphate. The GPD1 protein was expressed in Escherichia coli, and purified as a fusion protein with glutathione S-transferase. Here we, report the apoenzyme structure of GPD1 determined by multiwavelength, anomalous diffraction phasing, and other complex structures with small, molecules (NAD+ and DHAP) by the molecular replacement method. This enzyme, structure is organized into two distinct domains, the N-terminal, eight-stranded beta-sheet sandwich domain and the C-terminal helical, substrate-binding domain. An electrophilic catalytic mechanism by the, epsilon-NH3+ group of Lys204 is proposed on the basis of the structural, analyses. In addition, the inhibitory effects of zinc and sulfate on GPDHs, are assayed and discussed.
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<StructureSection load='1x0x' size='340' side='right'caption='[[1x0x]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1x0x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X0X FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x0x OCA], [https://pdbe.org/1x0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x0x RCSB], [https://www.ebi.ac.uk/pdbsum/1x0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x0x ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/GPDA_HUMAN GPDA_HUMAN] Defects in GPD1 are a cause of hypertriglyceridemia, transient infantile (HTGTI) [MIM:[https://omim.org/entry/614480 614480]. An autosomal recessive disorder characterized by onset of moderate to severe transient hypertriglyceridemia in infancy that normalizes with age. The hypertriglyceridemia is associated with hepatomegaly, moderately elevated transaminases, persistent fatty liver, and the development of hepatic fibrosis.<ref>PMID:22226083</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/GPDA_HUMAN GPDA_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x0/1x0x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x0x ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1X0X is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 and NAD as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycerol-3-phosphate_dehydrogenase_(NAD(+)) Glycerol-3-phosphate dehydrogenase (NAD(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.8 1.1.1.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1X0X OCA].
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*[[Glycerol-3-phosphate dehydrogenase 3D structures|Glycerol-3-phosphate dehydrogenase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structures of human glycerol 3-phosphate dehydrogenase 1 (GPD1)., Ou X, Ji C, Han X, Zhao X, Li X, Mao Y, Wong LL, Bartlam M, Rao Z, J Mol Biol. 2006 Mar 31;357(3):858-69. Epub 2006 Jan 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16460752 16460752]
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__TOC__
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[[Category: Glycerol-3-phosphate dehydrogenase (NAD(+))]]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ou, X.]]
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[[Category: Ou X]]
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[[Category: Rao, Z.]]
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[[Category: Rao Z]]
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[[Category: NAD]]
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[[Category: SO4]]
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[[Category: co-enzyme]]
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[[Category: gpd1]]
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[[Category: nad]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:57:34 2007''
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Current revision

Co-Structure of Homo Sapiens Glycerol-3-Phosphate Dehydrogenase 1 complex with NAD

PDB ID 1x0x

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