1xaw

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1xaw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xaw, resolution 1.45&Aring;" /> '''crystal structure o...)
Current revision (08:49, 14 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1xaw.gif|left|200px]]<br />
 
-
<applet load="1xaw" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1xaw, resolution 1.45&Aring;" />
 
-
'''crystal structure of the cytoplasmic distal C-terminal domain of occludin'''<br />
 
-
==Overview==
+
==crystal structure of the cytoplasmic distal C-terminal domain of occludin==
-
Occludin is a transmembrane protein localized at tight junctions whose, functions are complex yet poorly understood. Current evidence supports a, role for occludin in both the formation of the paracellular barrier and in, cell signaling. While the N-terminal extracellular domains of occludin, mediate homotypic adhesion, the distal C-terminal cytoplasmic domain of, occludin controls protein targeting and endocytosis. The C terminus can, also bind to the scaffolding proteins ZO-1, ZO-2, ZO-3, cingulin, the, membrane trafficking protein VAP33, and the cytoskeletal protein F-actin, suggesting an important role for this domain. This domain is highly, homologous to an important functional domain in the C terminus of the ELL, family of RNA polymerase II transcription factors. To explore the function, of occludin, we determined the high-resolution crystal structure of its, C-terminal distal cytoplasmic domain. The structure comprises three, helices that form two separate anti-parallel coiled-coils and a loop that, packs tightly against one of the coiled-coils. Using in vitro binding, studies and site-directed mutagenesis, we have identified a large, positively charged surface that contains the binding site for ZO-1, and, this surface is required for proper localization of occludin to cell-cell, junctions. On the basis of sequence conservation, we predict that occludin, domains from different species and the C-terminal domain of the ELL, transcription factors share a very similar structure. Our results provide, a model to further test the function of occludin and its binding to other, proteins.
+
<StructureSection load='1xaw' size='340' side='right'caption='[[1xaw]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1xaw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XAW FirstGlance]. <br>
-
1XAW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XAW OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xaw OCA], [https://pdbe.org/1xaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xaw RCSB], [https://www.ebi.ac.uk/pdbsum/1xaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xaw ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
Structure of the conserved cytoplasmic C-terminal domain of occludin: identification of the ZO-1 binding surface., Li Y, Fanning AS, Anderson JM, Lavie A, J Mol Biol. 2005 Sep 9;352(1):151-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16081103 16081103]
+
== Disease ==
 +
[https://www.uniprot.org/uniprot/OCLN_HUMAN OCLN_HUMAN] Defects in OCLN are the cause of band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:[https://omim.org/entry/251290 251290]; also known as pseudo-TORCH syndrome. BLCPMG is a neurologic disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications, and severe developmental delay.<ref>PMID:20727516</ref>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/OCLN_HUMAN OCLN_HUMAN] May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.<ref>PMID:19114660</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xa/1xaw_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xaw ConSurf].
 +
<div style="clear:both"></div>
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Anderson, J.M.]]
+
[[Category: Anderson JM]]
-
[[Category: Fanning, A.S.]]
+
[[Category: Fanning AS]]
-
[[Category: Lavie, A.]]
+
[[Category: Lavie A]]
-
[[Category: Li, Y.]]
+
[[Category: Li Y]]
-
[[Category: coiled-coil]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:02:54 2007''
+

Current revision

crystal structure of the cytoplasmic distal C-terminal domain of occludin

PDB ID 1xaw

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xaw"
Personal tools