1xcg

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(New page: 200px<br /> <applet load="1xcg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xcg, resolution 2.50&Aring;" /> '''Crystal Structure o...)
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[[Image:1xcg.gif|left|200px]]<br />
 
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<applet load="1xcg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xcg, resolution 2.50&Aring;" />
 
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'''Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF'''<br />
 
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==Overview==
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==Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF==
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Calcium sensitization in smooth muscle is mediated by the RhoA GTPase, activated by hitherto unspecified nucleotide exchange factors (GEFs), acting downstream of Galphaq/Galpha(12/13) trimeric G proteins. Here, we, show that at least one potential GEF, the PDZRhoGEF, is present in smooth, muscle, and its isolated DH/PH fragment induces calcium sensitization in, the absence of agonist-mediated signaling. In vitro, the fragment shows, high selectivity for the RhoA GTPase. Full-length fragment is required for, the nucleotide exchange, as the isolated DH domain enhances it only, marginally. We crystallized the DH/PH fragment of PDZRhoGEF in complex, with nonprenylated human RhoA and determined the structure at 2.5 A, resolution. The refined molecular model reveals that the mutual, disposition of the DH and PH domains is significantly different from other, previously described complexes involving DH/PH tandems, and that the PH, domain interacts with RhoA in a unique mode. The DH domain makes several, specific interactions with RhoA residues not conserved among other Rho, family members, suggesting the molecular basis for the observed, specificity.
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<StructureSection load='1xcg' size='340' side='right'caption='[[1xcg]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xcg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcg OCA], [https://pdbe.org/1xcg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcg RCSB], [https://www.ebi.ac.uk/pdbsum/1xcg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARHGB_HUMAN ARHGB_HUMAN] May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcg ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1XCG is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XCG OCA].
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*[[Rho GTPase 3D structures|Rho GTPase 3D structures]]
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*[[Rho guanine nucleotide exchange factor 3D structures|Rho guanine nucleotide exchange factor 3D structures]]
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==Reference==
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__TOC__
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The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle., Derewenda U, Oleksy A, Stevenson AS, Korczynska J, Dauter Z, Somlyo AP, Otlewski J, Somlyo AV, Derewenda ZS, Structure. 2004 Nov;12(11):1955-65. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15530360 15530360]
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Dauter, Z.]]
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[[Category: Dauter Z]]
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[[Category: Derewenda, U.]]
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[[Category: Derewenda U]]
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[[Category: Derewenda, Z.S.]]
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[[Category: Derewenda ZS]]
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[[Category: Korczynska, J.]]
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[[Category: Korczynska J]]
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[[Category: Oleksy, A.]]
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[[Category: Oleksy A]]
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[[Category: Otlewski, J.]]
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[[Category: Otlewski J]]
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[[Category: Somlyo, A.P.]]
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[[Category: Somlyo AP]]
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[[Category: Somlyo, A.V.]]
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[[Category: Somlyo AV]]
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[[Category: Stevenson, A.S.]]
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[[Category: Stevenson AS]]
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[[Category: x-ray crystallography; regulation of rhoa gtpase; protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:03:29 2007''
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Current revision

Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF

PDB ID 1xcg

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