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1xcr

From Proteopedia

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(New page: 200px<br /> <applet load="1xcr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xcr, resolution 1.70&Aring;" /> '''Crystal Structure o...)
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[[Image:1xcr.gif|left|200px]]<br />
 
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<applet load="1xcr" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xcr, resolution 1.70&Aring;" />
 
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'''Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold'''<br />
 
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==Overview==
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==Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold==
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The human protein PTD012 is the longer product of an alternatively spliced, gene and was described to be localized in the nucleus. The X-ray structure, analysis at 1.7 A resolution of PTD012 through SAD phasing reveals a, monomeric protein and a novel fold. The shorter splice form was also, studied and appears to be unfolded and non-functional. The structure of, PTD012 displays an alphabetabetaalpha four-layer topology. A metal ion, residing between the central beta-sheets is partially coordinated by three, histidine residues. X-ray absorption near-edge structure (XANES) analysis, identifies the PTD012-bound ion as Zn(2+). Tetrahedral coordination of the, ion is completed by the carboxylate oxygen atom of an acetate molecule, taken up from the crystallization buffer. The binding of Zn(2+) to PTD012, is reminiscent of zinc-containing enzymes such as carboxypeptidase, carbonic anhydrase, and beta-lactamase. Biochemical assays failed to, demonstrate any of these enzyme activities in PTD012. However, PTD012, exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.
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<StructureSection load='1xcr' size='340' side='right'caption='[[1xcr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xcr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XCR FirstGlance]. <br>
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1XCR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XCR OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xcr OCA], [https://pdbe.org/1xcr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xcr RCSB], [https://www.ebi.ac.uk/pdbsum/1xcr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xcr ProSAT]</span></td></tr>
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Crystal structure of Homo sapiens PTD012 reveals a zinc-containing hydrolase fold., Manjasetty BA, Bussow K, Fieber-Erdmann M, Roske Y, Gobom J, Scheich C, Gotz F, Niesen FH, Heinemann U, Protein Sci. 2006 Apr;15(4):914-20. Epub 2006 Mar 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16522806 16522806]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CK054_HUMAN CK054_HUMAN] Exhibits ester hydrolase activity on the substrate p-nitrophenyl acetate.<ref>PMID:16522806</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xc/1xcr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xcr ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Buessow, K.]]
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[[Category: Buessow K]]
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[[Category: Fieber-Erdmann, M.]]
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[[Category: Fieber-Erdmann M]]
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[[Category: Goetz, F.]]
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[[Category: Goetz F]]
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[[Category: Heinemann, U.]]
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[[Category: Heinemann U]]
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[[Category: Manjasetty, B.A.]]
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[[Category: Manjasetty BA]]
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[[Category: Roske, Y.]]
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[[Category: Roske Y]]
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[[Category: ACY]]
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[[Category: ZN]]
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[[Category: acetate buffer]]
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[[Category: splice variant]]
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[[Category: structural genomics]]
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[[Category: zinc-containing fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:03:35 2007''
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Current revision

Crystal Structure of Longer Splice Variant of PTD012 from Homo sapiens reveals a novel Zinc-containing fold

PDB ID 1xcr

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