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| - | [[Image:1ttw.gif|left|200px]] | |
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| - | <!--
| + | ==Crystal structure of the Yersinia Pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2== |
| - | The line below this paragraph, containing "STRUCTURE_1ttw", creates the "Structure Box" on the page.
| + | <StructureSection load='1ttw' size='340' side='right'caption='[[1ttw]], [[Resolution|resolution]] 2.38Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1ttw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_enterocolitica Yersinia enterocolitica] and [https://en.wikipedia.org/wiki/Yersinia_pestis_CO92 Yersinia pestis CO92]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TTW FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38Å</td></tr> |
| - | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ttw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ttw OCA], [https://pdbe.org/1ttw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ttw RCSB], [https://www.ebi.ac.uk/pdbsum/1ttw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ttw ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1ttw| PDB=1ttw | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''Crystal structure of the Yersinia Pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2'''
| + | [https://www.uniprot.org/uniprot/Q7BTX0_YERPE Q7BTX0_YERPE] |
| - | | + | __TOC__ |
| - | | + | </StructureSection> |
| - | ==Overview== | + | [[Category: Large Structures]] |
| - | Pathogenic Yersinia species use a type III secretion system to inject cytotoxic effector proteins directly into the cytosol of mammalian cells, where they neutralize the innate immune response by interfering with the signal-transduction pathways that control phagocytosis and inflammation. To be exported efficiently, some effectors must transiently associate with cognate cytoplasmic secretion chaperones. SycH is the chaperone for YopH, a potent eukaryotic-like protein tyrosine phosphatase that is essential for virulence. SycH also binds two negative regulators of type III secretion, YscM1 and YscM2, both of which share significant sequence homology with the chaperone-binding domain of YopH. Here, the structure of a complex between SycH and a stable fragment of YscM2 that was designed on the basis of limited proteolysis experiments is presented. The overall fold of SycH is very similar to the structures of other homodimeric secretion chaperones that have been determined to date. YscM2 wraps around SycH in an extended fashion, with some secondary but no tertiary structure, assuming a conformation distinct from the globular fold that it is predicted to adopt in the absence of SycH.
| + | [[Category: Yersinia enterocolitica]] |
| - | | + | [[Category: Yersinia pestis CO92]] |
| - | ==About this Structure== | + | [[Category: Phan J]] |
| - | 1TTW is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Yersinia_pestis_co92 Yersinia pestis co92]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TTW OCA].
| + | [[Category: Tropea JE]] |
| - | | + | [[Category: Waugh DS]] |
| - | ==Reference== | + | |
| - | Structure of the Yersinia pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2., Phan J, Tropea JE, Waugh DS, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1591-9. Epub 2004, Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15333930 15333930]
| + | |
| - | [[Category: Protein complex]] | + | |
| - | [[Category: Yersinia pestis co92]] | + | |
| - | [[Category: Phan, J.]] | + | |
| - | [[Category: Tropea, J E.]] | + | |
| - | [[Category: Waugh, D S.]] | + | |
| - | [[Category: Chaperone]] | + | |
| - | [[Category: Type iii secretion]] | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:21:25 2008''
| + | |
