1txk

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[[Image:1txk.gif|left|200px]]
 
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==Crystal structure of Escherichia coli OpgG==
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The line below this paragraph, containing "STRUCTURE_1txk", creates the "Structure Box" on the page.
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<StructureSection load='1txk' size='340' side='right'caption='[[1txk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1txk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_1txk| PDB=1txk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txk OCA], [https://pdbe.org/1txk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txk RCSB], [https://www.ebi.ac.uk/pdbsum/1txk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txk ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of Escherichia coli OpgG'''
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== Function ==
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[https://www.uniprot.org/uniprot/OPGG_ECOLI OPGG_ECOLI] Involved in the biosynthesis of osmoregulated periplasmic glucans (OPGs).[HAMAP-Rule:MF_01069]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Osmoregulated periplasmic glucans (OPGs) G protein (OpgG) is required for OPGs biosynthesis. OPGs from Escherichia coli are branched glucans, with a backbone of beta-1,2 glucose units and with branches attached by beta-1,6 linkages. In Proteobacteria, OPGs are involved in osmoprotection, biofilm formation, virulence and resistance to antibiotics. Despite their important biological implications, enzymes synthesizing OPGs are poorly characterized. Here, we report the 2.5 A crystal structure of OpgG from E.coli. The structure was solved using a selenemethionine derivative of OpgG and the multiple anomalous diffraction method (MAD). The protein is composed of two beta-sandwich domains connected by one turn of 3(10) helix. The N-terminal domain (residues 22-388) displays a 25-stranded beta-sandwich fold found in several carbohydrate-related proteins. It exhibits a large cleft comprising many aromatic and acidic residues. This putative binding site shares some similarities with enzymes such as galactose mutarotase and glucodextranase, suggesting a potential catalytic role for this domain in OPG synthesis. On the other hand, the C-terminal domain (residues 401-512) has a seven-stranded immunoglobulin-like beta-sandwich fold, found in many proteins where it is mainly implicated in interactions with other molecules. The structural data suggest that OpgG is an OPG branching enzyme in which the catalytic activity is located in the large N-terminal domain and controlled via the smaller C-terminal domain.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txk_consurf.spt"</scriptWhenChecked>
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1TXK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXK OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural analysis of Escherichia coli OpgG, a protein required for the biosynthesis of osmoregulated periplasmic glucans., Hanoulle X, Rollet E, Clantin B, Landrieu I, Odberg-Ferragut C, Lippens G, Bohin JP, Villeret V, J Mol Biol. 2004 Sep 3;342(1):195-205. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15313617 15313617]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txk ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bohin, J P.]]
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[[Category: Bohin JP]]
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[[Category: Clantin, B.]]
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[[Category: Clantin B]]
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[[Category: Hanoulle, X.]]
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[[Category: Hanoulle X]]
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[[Category: Landrieu, I.]]
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[[Category: Landrieu I]]
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[[Category: Lippens, G.]]
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[[Category: Lippens G]]
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[[Category: Odberg-Ferragut, C.]]
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[[Category: Odberg-Ferragut C]]
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[[Category: Rollet, E.]]
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[[Category: Rollet E]]
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[[Category: Villeret, V.]]
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[[Category: Villeret V]]
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[[Category: Beta-sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:29:23 2008''
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Current revision

Crystal structure of Escherichia coli OpgG

PDB ID 1txk

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