9dsm
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of SSNA-1(R18E/R20E/Q98E) filaments== | |
| + | <StructureSection load='9dsm' size='340' side='right'caption='[[9dsm]], [[Resolution|resolution]] 4.55Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9dsm]] is a 32 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9DSM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9DSM FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.55Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9dsm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9dsm OCA], [https://pdbe.org/9dsm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9dsm RCSB], [https://www.ebi.ac.uk/pdbsum/9dsm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9dsm ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q5F4U5_CAEEL Q5F4U5_CAEEL] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | SSNA1 is a fibrillar protein involved in dynamic microtubule remodeling, including nucleation, co-polymerization, and microtubule branching. The underlying molecular mechanism has remained unclear due to a lack of structural information. Here, we determine the cryo-EM structure of C.elegans SSNA-1 at 4.55-A resolution and evaluate its role in embryonic development. We find that SSNA-1 forms an anti-parallel coiled-coil, with self-assembly facilitated by an overhang of 16 C-terminal residues that form a triple-stranded helical junction. The microtubule-binding region is within the triple-stranded junction, suggesting that self-assembly of SSNA-1 creates hubs for effective microtubule interaction. Genetical analysis elucidates that SSNA-1 deletion significantly reduces embryonic viability, and causes multipolar spindles during cell division. Interestingly, impairing SSNA-1 self-assembly has a comparable effect on embryonic viability as the knockout strain. Our study provides molecular insights into SSNA-1's self-assembly and its role in microtubule binding and cell division regulation through centriole stability. | ||
| - | + | Structural insights into SSNA1 self-assembly and its microtubule binding for centriole maintenance.,Agostini L, Pfister JA, Basnet N, Ding J, Zhang R, Biertumpfel C, O'Connell KF, Mizuno N Nat Commun. 2025 Aug 13;16(1):7512. doi: 10.1038/s41467-025-62696-9. PMID:40804232<ref>PMID:40804232</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9dsm" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Caenorhabditis elegans]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Agostini L]] | ||
| + | [[Category: Biertumpfel C]] | ||
| + | [[Category: Mizuno N]] | ||
Current revision
Cryo-EM structure of SSNA-1(R18E/R20E/Q98E) filaments
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