1u5o

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[[Image:1u5o.jpg|left|200px]]
 
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==Structure of the D23A mutant of the nuclear transport carrier NTF2==
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The line below this paragraph, containing "STRUCTURE_1u5o", creates the "Structure Box" on the page.
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<StructureSection load='1u5o' size='340' side='right'caption='[[1u5o]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1u5o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U5O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u5o OCA], [https://pdbe.org/1u5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u5o RCSB], [https://www.ebi.ac.uk/pdbsum/1u5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u5o ProSAT]</span></td></tr>
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{{STRUCTURE_1u5o| PDB=1u5o | SCENE= }}
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</table>
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== Function ==
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'''Structure of the D23A mutant of the nuclear transport carrier NTF2'''
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[https://www.uniprot.org/uniprot/NTF2_RAT NTF2_RAT] Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Nuclear transport carriers interact with proteins of the nuclear pore complex (NPC) to transport their cargo across the nuclear envelope. One such carrier is nuclear transport factor 2 (NTF2), whose import cargo is the small GTPase Ran. A domain highly homologous to the small NTF2 protein (14kDa) is also found in a number of additional proteins, which together make up the NTF2 domain containing superfamily of proteins. Using structural, computational and biochemical analysis we have identified a functional site that is present throughout this superfamily, and our results indicate that this site functions as an NPC binding site in NTF2. Previously we showed that a D23A mutant of NTF2 exhibits increased affinity for the NPC. The mechanism of this mutation, however, was unknown as this region of NTF2 had not been implicated in binding to NPC proteins. Here we show that the D23A mutation in NTF2 does not result in gross structural changes affecting other known NPC binding sites. Instead, the D23 residue is located in an evolutionarily important region in the NTF2 domain containing superfamily, that in NTF2, is involved in binding to the NPC.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u5/1u5o_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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1U5O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U5O OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u5o ConSurf].
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Computational and biochemical identification of a nuclear pore complex binding site on the nuclear transport carrier NTF2., Cushman I, Bowman BR, Sowa ME, Lichtarge O, Quiocho FA, Moore MS, J Mol Biol. 2004 Nov 19;344(2):303-10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15522285 15522285]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Bowman BR]]
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[[Category: Bowman, B R.]]
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[[Category: Cushman I]]
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[[Category: Cushman, I.]]
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[[Category: Lichtarge O]]
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[[Category: Lichtarge, O.]]
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[[Category: Moore MS]]
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[[Category: Moore, M S.]]
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[[Category: Quiocho FA]]
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[[Category: Quiocho, F A.]]
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[[Category: Sowa ME]]
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[[Category: Sowa, M E.]]
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[[Category: Ntf2 mutant d23a]]
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[[Category: Nuclear transport protein]]
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[[Category: Transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:47:15 2008''
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Current revision

Structure of the D23A mutant of the nuclear transport carrier NTF2

PDB ID 1u5o

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