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| - | [[Image:1u7e.gif|left|200px]] | + | #REDIRECT [[3fhi]] This PDB entry is obsolete and replaced by 3fhi |
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| - | {{STRUCTURE_1u7e| PDB=1u7e | SCENE= }}
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| - | '''The crystal structure of a Protein Kinase A complex'''
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| - | ==Overview==
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| - | The 2.0-angstrom structure of the cyclic adenosine monophosphate (cAMP)-dependent protein kinase (PKA) catalytic subunit bound to a deletion mutant of a regulatory subunit (RIalpha) defines a previously unidentified extended interface. The complex provides a molecular mechanism for inhibition of PKA and suggests how cAMP binding leads to activation. The interface defines the large lobe of the catalytic subunit as a stable scaffold where Tyr247 in the G helix and Trp196 in the phosphorylated activation loop serve as anchor points for binding RIalpha. These residues compete with cAMP for the phosphate binding cassette in RIalpha. In contrast to the catalytic subunit, RIalpha undergoes major conformational changes when the complex is compared with cAMP-bound RIalpha. The inhibitor sequence docks to the active site, whereas the linker, also disordered in free RIalpha, folds across the extended interface. The beta barrel of cAMP binding domain A, which is the docking site for cAMP, remains largely intact in the complex, whereas the helical subdomain undergoes major reorganization.
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| - | ==About this Structure==
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| - | 1U7E is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7E OCA].
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| - | ==Reference==
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| - | Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA., Kim C, Xuong NH, Taylor SS, Science. 2005 Feb 4;307(5710):690-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15692043 15692043]
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| - | [[Category: Bos taurus]]
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| - | [[Category: Mus musculus]]
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| - | [[Category: Non-specific serine/threonine protein kinase]]
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| - | [[Category: Protein complex]]
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| - | [[Category: Kim, C.]]
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| - | [[Category: Taylor, S S.]]
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| - | [[Category: Xuong, N H.]]
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| - | [[Category: Amp-pnp]]
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| - | [[Category: Camp]]
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| - | [[Category: Camp dependent protein kinase]]
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| - | [[Category: Pk regulation]]
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| - | [[Category: Protein-protein complx]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:50:54 2008''
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