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1u7g

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[[Image:1u7g.jpg|left|200px]]
 
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==Crystal Structure of Ammonia Channel AmtB from E. Coli==
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The line below this paragraph, containing "STRUCTURE_1u7g", creates the "Structure Box" on the page.
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<StructureSection load='1u7g' size='340' side='right'caption='[[1u7g]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1u7g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U7G FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NH3:AMMONIA'>NH3</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene></td></tr>
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{{STRUCTURE_1u7g| PDB=1u7g | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u7g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u7g OCA], [https://pdbe.org/1u7g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u7g RCSB], [https://www.ebi.ac.uk/pdbsum/1u7g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u7g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u7/1u7g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u7g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.
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'''Crystal Structure of Ammonia Channel AmtB from E. Coli'''
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Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.,Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM Science. 2004 Sep 10;305(5690):1587-94. PMID:15361618<ref>PMID:15361618</ref>
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==Overview==
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The first structure of an ammonia channel from the Amt/MEP/Rh protein superfamily, determined to 1.35 angstrom resolution, shows it to be a channel that spans the membrane 11 times. Two structurally similar halves span the membrane with opposite polarity. Structures with and without ammonia or methyl ammonia show a vestibule that recruits NH4+/NH3, a binding site for NH4+, and a 20 angstrom-long hydrophobic channel that lowers the NH4+ pKa to below 6 and conducts NH3. Favorable interactions for NH3 are seen within the channel and use conserved histidines. Reconstitution of AmtB into vesicles shows that AmtB conducts uncharged NH3.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1U7G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7G OCA].
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</div>
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<div class="pdbe-citations 1u7g" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A., Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM, Science. 2004 Sep 10;305(5690):1587-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15361618 15361618]
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: III, J O.Connell.]]
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[[Category: Khademi S]]
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[[Category: Khademi, S.]]
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[[Category: Miercke LJW]]
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[[Category: Miercke, L J.W.]]
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[[Category: O'Connell III J]]
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[[Category: Remis, J.]]
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[[Category: Remis J]]
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[[Category: Robles-Colmenares, Y.]]
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[[Category: Robles-Colmenares Y]]
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[[Category: Stroud, R M.]]
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[[Category: Stroud RM]]
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[[Category: Ammonia channnel]]
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[[Category: Membrane protein]]
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[[Category: Rh protein]]
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[[Category: Right handed helical bundle]]
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[[Category: Transmembrane helice]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:51:04 2008''
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Current revision

Crystal Structure of Ammonia Channel AmtB from E. Coli

PDB ID 1u7g

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