1u7p

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[[Image:1u7p.gif|left|200px]]
 
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==X-ray Crystal Structure of the Hypothetical Phosphotyrosine Phosphatase MDP-1 of the Haloacid Dehalogenase Superfamily==
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The line below this paragraph, containing "STRUCTURE_1u7p", creates the "Structure Box" on the page.
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<StructureSection load='1u7p' size='340' side='right'caption='[[1u7p]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1u7p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U7P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=WO4:TUNGSTATE(VI)ION'>WO4</scene></td></tr>
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{{STRUCTURE_1u7p| PDB=1u7p | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u7p OCA], [https://pdbe.org/1u7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u7p RCSB], [https://www.ebi.ac.uk/pdbsum/1u7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u7p ProSAT]</span></td></tr>
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</table>
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'''X-ray Crystal Structure of the Hypothetical Phosphotyrosine Phosphatase MDP-1 of the Haloacid Dehalogenase Superfamily'''
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== Function ==
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[https://www.uniprot.org/uniprot/MGDP1_MOUSE MGDP1_MOUSE] Magnesium-dependent phosphatase which may act as a tyrosine phosphatase.<ref>PMID:10889041</ref> <ref>PMID:11601995</ref> <ref>PMID:15461449</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The haloacid dehalogenase (HAD) superfamily is comprised of structurally homologous enzymes that share several conserved sequence motifs (loops I-IV) in their active site. The majority of HAD members are phosphohydrolases and may be divided into three subclasses depending on domain organization. In classes I and II, a mobile "cap" domain reorients upon substrate binding, closing the active site to bulk solvent. Members of the third class lack this additional domain. Herein, we report the 1.9 A X-ray crystal structures of a member of the third subclass, magnesium-dependent phosphatase-1 (MDP-1) both in its unliganded form and with the product analogue, tungstate, bound to the active site. The secondary structure of MDP-1 is similar to that of the "core" domain of other type I and type II HAD members with the addition of a small, 28-amino acid insert that does not close down to exclude bulk solvent in the presence of ligand. In addition, the monomeric oligomeric state of MDP-1 does not allow the participation of a second subunit in the formation and solvent protection of the active site. The binding sites for the phosphate portion of the substrate and Mg(II) cofactor are also similar to those of other HAD members, with all previously observed contacts conserved. Unlike other subclass III HAD members, MDP-1 appears to be equally able to dephosphorylate phosphotyrosine and closed-ring phosphosugars. Modeling of possible substrates in the active site of MDP-1 reveals very few potential interactions with the substrate leaving group. The mapping of conserved residues in sequences of MDP-1 from different eukaryotic organisms reveals that they colocalize to a large region on the surface of the protein outside the active site. This observation combined with the modeling studies suggests that the target of MDP-1 is most likely a phosphotyrosine in an unknown protein rather than a small sugar-based substrate.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u7/1u7p_consurf.spt"</scriptWhenChecked>
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1U7P is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U7P OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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X-ray crystal structure of the hypothetical phosphotyrosine phosphatase MDP-1 of the haloacid dehalogenase superfamily., Peisach E, Selengut JD, Dunaway-Mariano D, Allen KN, Biochemistry. 2004 Oct 12;43(40):12770-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15461449 15461449]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u7p ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Allen KN]]
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[[Category: Allen, K N.]]
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[[Category: Dunaway-Mariano D]]
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[[Category: Dunaway-Mariano, D.]]
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[[Category: Peisach E]]
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[[Category: Peisach, E.]]
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[[Category: Selengut JD]]
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[[Category: Selengut, J D.]]
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[[Category: Aspartate nucleophile]]
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[[Category: Class iii]]
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[[Category: Enzyme evolution]]
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[[Category: Had superfamily]]
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[[Category: Phosphoryl transfer]]
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[[Category: Phosphotyrosine phosphatase]]
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[[Category: Structural enzymology]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:51:45 2008''
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Current revision

X-ray Crystal Structure of the Hypothetical Phosphotyrosine Phosphatase MDP-1 of the Haloacid Dehalogenase Superfamily

PDB ID 1u7p

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