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| - | [[Image:1u8d.gif|left|200px]] | + | #REDIRECT [[4fe5]] This PDB entry is obsolete and replaced by 4fe5 |
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| - | {{STRUCTURE_1u8d| PDB=1u8d | SCENE= }}
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| - | '''Guanine riboswitch bound to hypoxanthine'''
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| - | ==Overview==
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| - | Riboswitches are genetic regulatory elements found in the 5' untranslated region of messenger RNA that act in the absence of protein cofactors. They are broadly distributed across bacteria and account for the regulation of more than 2% of all genes in Bacillus subtilis, underscoring their importance in the control of cellular metabolism. The 5' untranslated region of many mRNAs of genes involved in purine metabolism and transport contain a guanine-responsive riboswitch that directly binds guanine, hypoxanthine or xanthine to terminate transcription. Here we report the crystal structure at 1.95 A resolution of the purine-binding domain of the guanine riboswitch from the xpt-pbuX operon of B. subtilis bound to hypoxanthine, a prevalent metabolite in the bacterial purine salvage pathway. This structure reveals a complex RNA fold involving several phylogenetically conserved nucleotides that create a binding pocket that almost completely envelops the ligand. Hypoxanthine functions to stabilize this structure and to promote the formation of a downstream transcriptional terminator element, thereby providing a mechanism for directly repressing gene expression in response to an increase in intracellular concentrations of metabolite.
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| - | ==About this Structure==
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| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U8D OCA].
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| - | ==Reference==
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| - | Structure of a natural guanine-responsive riboswitch complexed with the metabolite hypoxanthine., Batey RT, Gilbert SD, Montange RK, Nature. 2004 Nov 18;432(7015):411-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15549109 15549109]
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| - | [[Category: Batey, R T.]]
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| - | [[Category: Gilbert, S D.]]
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| - | [[Category: Montange, R K.]]
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| - | [[Category: Base quadruple]]
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| - | [[Category: Base triple]]
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| - | [[Category: Double helix]]
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| - | [[Category: Mrna]]
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| - | [[Category: Rna-ligand complex]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 10:53:22 2008''
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