1y2a

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(New page: 200px<br /> <applet load="1y2a" size="450" color="white" frame="true" align="right" spinBox="true" caption="1y2a, resolution 2.20&Aring;" /> '''Structure of mammal...)
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[[Image:1y2a.gif|left|200px]]<br />
 
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<applet load="1y2a" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1y2a, resolution 2.20&Aring;" />
 
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'''Structure of mammalian importin bound to the non-classical PLSCR1-NLS'''<br />
 
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==Overview==
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==Structure of mammalian importin bound to the non-classical PLSCR1-NLS==
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Nuclear import of proteins containing a classical nuclear localization, signal (NLS) is an energy-dependent process that requires the heterodimer, importin alpha/beta. Three to six basic contiguous arginine/lysine, residues characterize a classical NLS and are thought to form a basic, patch on the surface of the import cargo. In this study, we have, characterized the NLS of phospholipid scramblase 1 (PLSCR1), a, lipid-binding protein that enters the nucleus via the nonclassical NLS, (257)GKISKHWTGI(266). This import sequence lacks a contiguous stretch of, positively charged residues, and it is enriched in hydrophobic residues., We have determined the 2.2 A crystal structure of a complex between the, PLSCR1 NLS and the armadillo repeat core of vertebrate importin alpha. Our, crystallographic analysis reveals that PLSCR1 NLS binds to armadillo, repeats 1-4 of importin alpha, but its interaction partially overlaps the, classical NLS binding site. Two PLSCR1 lysines occupy the canonical, positions indicated as P2 and P5. Moreover, we present in vivo evidence, that the critical lysine at position P2, which is essential in other known, NLS sequences, is dispensable in PLSCR1 NLS. Taken together, these data, provide insight into a novel nuclear localization signal that presents a, distinct motif for binding to importin alpha.
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<StructureSection load='1y2a' size='340' side='right'caption='[[1y2a]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1y2a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y2A FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y2a OCA], [https://pdbe.org/1y2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y2a RCSB], [https://www.ebi.ac.uk/pdbsum/1y2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y2a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IMA1_MOUSE IMA1_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/y2/1y2a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1y2a ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1Y2A is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1Y2A OCA].
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*[[Importin 3D structures|Importin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Phospholipid scramblase 1 contains a nonclassical nuclear localization signal with unique binding site in importin alpha., Chen MH, Ben-Efraim I, Mitrousis G, Walker-Kopp N, Sims PJ, Cingolani G, J Biol Chem. 2005 Mar 18;280(11):10599-606. Epub 2004 Dec 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15611084 15611084]
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[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Ben-Efraim I]]
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[[Category: Ben-Efraim, I.]]
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[[Category: Chen M-H]]
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[[Category: Chen, M.H.]]
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[[Category: Cingolani G]]
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[[Category: Cingolani, G.]]
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[[Category: Mitrousis G]]
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[[Category: Mitrousis, G.]]
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[[Category: Sims PJ]]
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[[Category: Sims, P.J.]]
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[[Category: Walker-Kopp N]]
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[[Category: Walker-Kopp, N.]]
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[[Category: armadillo repeat; protein:peptide complex; superhelix of helices]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:13:45 2007''
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Current revision

Structure of mammalian importin bound to the non-classical PLSCR1-NLS

PDB ID 1y2a

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