9eif
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 9eif is ON HOLD Authors: Gatreddi, S., Hausinger, R.P., Hu, J. Description: A broad-substrate spectrum lactate racemase A from Isosphaera pallida i...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==A broad-substrate spectrum lactate racemase A from Isosphaera pallida in complex with D-2-Hydroxyisovalerate== | |
| + | <StructureSection load='9eif' size='340' side='right'caption='[[9eif]], [[Resolution|resolution]] 1.65Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9eif]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Isosphaera_pallida Isosphaera pallida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9EIF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9EIF FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4EY:1-[(2R,3R,4S,5R)-3,4-BIS(OXIDANYL)-5-(PHOSPHONOOXYMETHYL)OXOLAN-2-YL]-5-METHANETHIOYL-PYRIDINE-3-CARBOTHIOIC+S-ACID'>4EY</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=VAD:DEAMINOHYDROXYVALINE'>VAD</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9eif FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9eif OCA], [https://pdbe.org/9eif PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9eif RCSB], [https://www.ebi.ac.uk/pdbsum/9eif PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9eif ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E8QWZ4_ISOPI E8QWZ4_ISOPI] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The LarA family consists of diverse racemases/epimerases that interconvert the diastereomers of a variety of alpha-hydroxyacids by using a nickel-pincer nucleotide (NPN) cofactor. The hidden redox reaction catalyzed by the NPN cofactor makes LarA enzymes attractive engineering targets for applications. However, how a LarA enzyme binds its natural substrate and recognizes different alpha-hydroxyacids has not been elucidated. Here, we report three high-resolution structures of the enzyme-substrate complexes of a broad-spectrum LarA enzyme from Isosphaera pallida (LarA (Ip) ). The substrate binding mode reveals an optimal orientation and distance between the hydride donor and acceptor, strongly supporting the proposed proton-coupled hydride transfer mechanism. The experimentally solved structures, together with the structural models of other LarA enzymes, allow us to identify the residues/structural elements critically involved in the interactions with different alpha-hydroxyacid substrates. Collectively, this work provides a critical structural basis for catalysis and substrate recognition of the diverse enzymes in the LarA family, thus building a foundation for enzyme engineering. | ||
| - | + | Structural Basis for Catalysis and Substrate Specificity of a LarA Racemase with a Broad Substrate Spectrum.,Gatreddi S, Urdiain-Arraiza J, Desguin B, Hausinger RP, Hu J bioRxiv [Preprint]. 2024 Nov 28:2024.11.28.625916. doi: , 10.1101/2024.11.28.625916. PMID:39651260<ref>PMID:39651260</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9eif" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Isosphaera pallida]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gatreddi S]] | ||
| + | [[Category: Hausinger RP]] | ||
| + | [[Category: Hu J]] | ||
Current revision
A broad-substrate spectrum lactate racemase A from Isosphaera pallida in complex with D-2-Hydroxyisovalerate
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