9knk
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of full-length PHA synthase (PhaC) from Aeromonas caviae== | |
| + | <StructureSection load='9knk' size='340' side='right'caption='[[9knk]], [[Resolution|resolution]] 3.29Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9knk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_caviae Aeromonas caviae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9KNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9KNK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.29Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9knk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9knk OCA], [https://pdbe.org/9knk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9knk RCSB], [https://www.ebi.ac.uk/pdbsum/9knk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9knk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/O32471_AERCA O32471_AERCA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Polyhydroxyalkanoate (PHA) is a biodegradable polyester that can serve as a promising alternative to petrochemical plastics, which present a serious source of pollution. PHA synthase (PhaC) is a key enzyme responsible for producing a wide variety of PHAs in microorganisms. Here, we present crystal structures of full-length PhaC from Aeromonas caviae, a high-performance PhaC employed for industrial use. The structure reveals an N-terminal helical domain that mediates head-to-head dimerization and stabilizes the C-terminal alpha/beta catalytic domain to form a tunnel that connects the catalytic center embedded inside the protein to the protein surface. We showed that this tunnel is a putative egress tunnel for the product PHA chain. Our results establish a fundamental understanding of the PhaC machinery that should lead to improvement of this enzyme in industrial applications. | ||
| - | + | Structures of Polyhydroxyalkanoate Synthase PhaC from Aeromonas caviae, Producing Biodegradable Plastics.,Chek MF, Kim SY, Mori T, Matsumoto K, Sato S, Hakoshima T Angew Chem Int Ed Engl. 2025 Apr 25:e202504626. doi: 10.1002/anie.202504626. PMID:40276819<ref>PMID:40276819</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9knk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Aeromonas caviae]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Chek MF]] | ||
| + | [[Category: Hakoshima T]] | ||
| + | [[Category: Kim SY]] | ||
| + | [[Category: Mori T]] | ||
Current revision
Crystal structure of full-length PHA synthase (PhaC) from Aeromonas caviae
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