1uqr

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[[Image:1uqr.gif|left|200px]]
 
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==Type II 3-dehydroquinate dehydratase (DHQase) from Actinobacillus pleuropneumoniae==
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The line below this paragraph, containing "STRUCTURE_1uqr", creates the "Structure Box" on the page.
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<StructureSection load='1uqr' size='340' side='right'caption='[[1uqr]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uqr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UQR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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{{STRUCTURE_1uqr| PDB=1uqr | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uqr OCA], [https://pdbe.org/1uqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uqr RCSB], [https://www.ebi.ac.uk/pdbsum/1uqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uqr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROQ_ACTPL AROQ_ACTPL] Catalyzes a trans-dehydration via an enolate intermediate (By similarity).[HAMAP-Rule:MF_00169]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uq/1uqr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uqr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the type II dehydroquinate dehydratase (DHQase) from Actinobacillus pleuropneumoniae, the third enzyme of the shikimate pathway, has been determined. Crystals diffracting to 1.7 A were obtained in space and on earth using the counter-diffusion technique. The structure was solved using molecular replacement and refined to high resolution. The overall structure of the dodecameric enzyme is described and compared with structures of DHQases from other bacteria. DHQases contain a flexible loop that presumably closes over the active site upon substrate binding. The enzyme can exist in an open or closed conformation. The present structure displays the open conformation, with a sulfate anion bound in the active site. The availability of this structure opens a route to structure-based antibiotics targetting this pathogenic bacterium.
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'''TYPE II 3-DEHYDROQUINATE DEHYDRATASE (DHQASE) FROM ACTINOBACILLUS PLEUROPNEUMONIAE'''
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Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae.,Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:14993670<ref>PMID:14993670</ref>
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==Overview==
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The structure of the type II dehydroquinate dehydratase (DHQase) from Actinobacillus pleuropneumoniae, the third enzyme of the shikimate pathway, has been determined. Crystals diffracting to 1.7 A were obtained in space and on earth using the counter-diffusion technique. The structure was solved using molecular replacement and refined to high resolution. The overall structure of the dodecameric enzyme is described and compared with structures of DHQases from other bacteria. DHQases contain a flexible loop that presumably closes over the active site upon substrate binding. The enzyme can exist in an open or closed conformation. The present structure displays the open conformation, with a sulfate anion bound in the active site. The availability of this structure opens a route to structure-based antibiotics targetting this pathogenic bacterium.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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1UQR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Actinobacillus_pleuropneumoniae Actinobacillus pleuropneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UQR OCA].
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</div>
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<div class="pdbe-citations 1uqr" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural study of the type II 3-dehydroquinate dehydratase from Actinobacillus pleuropneumoniae., Maes D, Gonzalez-Ramirez LA, Lopez-Jaramillo J, Yu B, De Bondt H, Zegers I, Afonina E, Garcia-Ruiz JM, Gulnik S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):463-71. Epub 2004, Feb 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14993670 14993670]
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*[[Dehydroquinase 3D structures|Dehydroquinase 3D structures]]
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[[Category: 3-dehydroquinate dehydratase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Actinobacillus pleuropneumoniae]]
[[Category: Actinobacillus pleuropneumoniae]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Afonina, E.]]
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[[Category: Afonina E]]
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[[Category: Bondt, H De.]]
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[[Category: De Bondt H]]
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[[Category: Garcia-Ruiz, J M.]]
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[[Category: Garcia-Ruiz JM]]
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[[Category: Gonzalez-Ramirez, L A.]]
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[[Category: Gonzalez-Ramirez LA]]
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[[Category: Gulnik, S.]]
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[[Category: Gulnik S]]
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[[Category: Lopez-Jaramillo, J.]]
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[[Category: Lopez-Jaramillo J]]
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[[Category: Maes, D.]]
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[[Category: Maes D]]
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[[Category: Yu, B.]]
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[[Category: Yu B]]
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[[Category: Zegers, I.]]
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[[Category: Zegers I]]
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[[Category: Aromatic amino acid biosynthesis]]
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[[Category: Lyase]]
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[[Category: Shikimate pathway]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:33:39 2008''
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Current revision

Type II 3-dehydroquinate dehydratase (DHQase) from Actinobacillus pleuropneumoniae

PDB ID 1uqr

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