1uty

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[[Image:1uty.gif|left|200px]]
 
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==Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)==
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The line below this paragraph, containing "STRUCTURE_1uty", creates the "Structure Box" on the page.
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<StructureSection load='1uty' size='340' side='right'caption='[[1uty]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uty]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bluetongue_virus_8 Bluetongue virus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UTY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uty FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uty OCA], [https://pdbe.org/1uty PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uty RCSB], [https://www.ebi.ac.uk/pdbsum/1uty PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uty ProSAT]</span></td></tr>
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{{STRUCTURE_1uty| PDB=1uty | SCENE= }}
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</table>
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== Function ==
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'''CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)'''
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[https://www.uniprot.org/uniprot/VNS2_BTV10 VNS2_BTV10] Single-stranded RNA-binding protein.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ut/1uty_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uty ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.
Bluetongue virus non-structural protein 2 belongs to a class of highly conserved proteins found in orbiviruses of the Reoviridae family. Non-structural protein 2 forms large multimeric complexes and localizes to cytoplasmic inclusions in infected cells. It is able to bind single-stranded RNA non-specifically, and it has been suggested that the protein is involved in the selection and condensation of the Bluetongue virus RNA segments prior to genome encapsidation. We have determined the x-ray structure of the N-terminal domain (sufficient for the RNA binding ability of non-structural protein 2) to 2.4 A resolution using anomalous scattering methods. Crystals of this apparently insoluble domain were obtained by in situ proteolysis of a soluble construct. The asymmetric unit shows two monomers related by non-crystallographic symmetry, with each monomer folded as a beta sandwich with a unique topology. The crystal structure reveals extensive monomer-monomer interactions, which explain the ability of the protein to self-assemble into large homomultimeric complexes. Of the entire surface area of the monomer, one-third is used to create the interfaces of the curved multimeric assembly observed in the x-ray structure. The structure reported here shows how the N-terminal domain would be able to bind single-stranded RNA non-specifically protecting the bound regions in a heterogeneous multimeric but not polymeric complex.
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==About this Structure==
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Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2.,Butan C, Van Der Zandt H, Tucker PA J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:15155766<ref>PMID:15155766</ref>
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1UTY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bluetongue_virus Bluetongue virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UTY OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2., Butan C, Van Der Zandt H, Tucker PA, J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15155766 15155766]
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</div>
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[[Category: Bluetongue virus]]
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<div class="pdbe-citations 1uty" style="background-color:#fffaf0;"></div>
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[[Category: RNA-directed RNA polymerase]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Butan, C.]]
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__TOC__
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[[Category: Tucker, P.]]
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</StructureSection>
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[[Category: Zandt, H Van Der.]]
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[[Category: Bluetongue virus 8]]
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[[Category: Rna binding protein]]
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[[Category: Large Structures]]
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[[Category: Viral protein]]
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[[Category: Butan C]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:41:08 2008''
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[[Category: Tucker P]]
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[[Category: Van Der zandt H]]

Current revision

Crystal structure of the RNA binding domain of Bluetongue virus non-structural protein 2(NS2)

PDB ID 1uty

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