9lgh
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 9lgh is ON HOLD Authors: Jo, E., Baek, Y., Ha, N.-C. Description: Cryo-EM structure of CotVW filament, bacillus subtilis endospore protein [[Catego...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Cryo-EM structure of CotVW filament, bacillus subtilis endospore protein== | |
| + | <StructureSection load='9lgh' size='340' side='right'caption='[[9lgh]], [[Resolution|resolution]] 3.32Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9lgh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9LGH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9LGH FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.32Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9lgh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9lgh OCA], [https://pdbe.org/9lgh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9lgh RCSB], [https://www.ebi.ac.uk/pdbsum/9lgh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9lgh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/COTV_BACSU COTV_BACSU] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The endospores of Bacillus subtilis are encased in a multilayered protective structure comprising core, cortex, inner and outer coats, and an outermost crust. Among the proteins required for crust formation, CotV and CotW are unique to B. subtilis and are hypothesized to be instrumental in maintaining spore surface integrity. However, their structural organization and functional mechanisms remain unclear. This study determined the cryogenic electron microscopy (cryo-EM) structure of the CotVW complex and revealed its filamentous helical architecture. Structural analysis showed that CotVW possesses a negatively charged surface that enables pH-dependent binding interactions. Specifically, at pH 6.0, CotVW engages in electrostatic interactions with histidine and positively charged residues, suggesting a potential regulatory mechanism influenced by the environmental pH. Our results elucidate the molecular basis of CotVW function in B. subtilis spore crust formation, highlighting its role in spore surface organization. This study advances our understanding of the spore coat architecture and may inform future research on bacterial spore resilience and structural adaptation. | ||
| - | + | Filamentous structure of the CotVW complex, the crust proteins of the Bacillus subtilis endospore.,Jo E, Kim D, Baek Y, Park M, Lee H, Ha NC J Biol Chem. 2025 Sep 11:110714. doi: 10.1016/j.jbc.2025.110714. PMID:40945727<ref>PMID:40945727</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9lgh" style="background-color:#fffaf0;"></div> |
| - | [[Category: Baek | + | == References == |
| - | [[Category: Ha | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Baek Y]] | ||
| + | [[Category: Ha N-C]] | ||
| + | [[Category: Jo E]] | ||
| + | [[Category: Kim D]] | ||
Current revision
Cryo-EM structure of CotVW filament, bacillus subtilis endospore protein
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