1uwi

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[[Image:1uwi.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF MUTATED BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS, WORKING AT MODERATE TEMPERATURE==
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The line below this paragraph, containing "STRUCTURE_1uwi", creates the "Structure Box" on the page.
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<StructureSection load='1uwi' size='340' side='right'caption='[[1uwi]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1uwi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UWI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UWI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uwi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uwi OCA], [https://pdbe.org/1uwi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uwi RCSB], [https://www.ebi.ac.uk/pdbsum/1uwi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uwi ProSAT]</span></td></tr>
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{{STRUCTURE_1uwi| PDB=1uwi | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BGAL_SACS2 BGAL_SACS2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uw/1uwi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uwi ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Comparison of the three-dimensional structure of hyperthermophilic and mesophilic beta-glycosidases shows differences in secondary structure composition. The enzymes from hyperthermophilic archaea have a significantly larger number of beta-strands arranged in supernumerary beta-sheets compared to mesophilic enzymes from bacteria and other organisms. Amino acid replacements designed to alter the structure of the supernumerary beta-strands were introduced by site directed mutagenesis into the sequence encoding the beta-glycosidase from Sulfolobus solfataricus. Most of the replacements caused almost complete loss of activity but some yielded enzyme variants whose activities were affected specifically at higher temperatures. Far-UV CD spectra recorded as a function of temperature for both wild type beta-glycosidase and mutant V349G, one of the mutants with reduced activity at higher temperatures, were similar, showing that the protein structure of the mutant was stable at the highest temperatures assayed. The properties of mutant V349G show a difference between thermostability (stability of the protein structure at high temperatures) and thermophilicity (optimal activity at high temperatures).
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'''CRYSTAL STRUCTURE OF MUTATED BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS, WORKING AT MODERATE TEMPERATURE'''
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Comparative study and mutational analysis of distinctive structural elements of hyperthermophilic enzymes.,Leon M, Isorna P, Menendez M, Sanz-Aparicio J, Polaina J Protein J. 2007 Sep;26(6):435-44. PMID:17503162<ref>PMID:17503162</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1uwi" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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1UWI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UWI OCA].
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*[[Galactosidase 3D structures|Galactosidase 3D structures]]
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[[Category: Beta-galactosidase]]
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== References ==
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[[Category: Single protein]]
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<references/>
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[[Category: Sulfolobus solfataricus]]
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__TOC__
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[[Category: Isorna, P.]]
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</StructureSection>
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[[Category: Polaina, J.]]
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[[Category: Large Structures]]
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[[Category: Sanz-Aparicio, J.]]
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[[Category: Saccharolobus solfataricus P2]]
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[[Category: Beta-glycosidase]]
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[[Category: Isorna P]]
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[[Category: Glycosidase]]
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[[Category: Polaina J]]
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[[Category: Hydrolase]]
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[[Category: Sanz-Aparicio J]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 11:46:41 2008''
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Current revision

CRYSTAL STRUCTURE OF MUTATED BETA-GLYCOSIDASE FROM SULFOLOBUS SOLFATARICUS, WORKING AT MODERATE TEMPERATURE

PDB ID 1uwi

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