9q8j
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==CryoEM structure of modified Turnip Yellows Virus devoid of minor capsid protein readthrough domain== | |
| + | <StructureSection load='9q8j' size='340' side='right'caption='[[9q8j]], [[Resolution|resolution]] 3.47Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9q8j]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Turnip_yellows_virus Turnip yellows virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9Q8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9Q8J FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.47Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9q8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9q8j OCA], [https://pdbe.org/9q8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9q8j RCSB], [https://www.ebi.ac.uk/pdbsum/9q8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9q8j ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/MCAPS_TYYVF MCAPS_TYYVF] Minor component of the viral capsid involved in aphid transmission and virus accumulation in the host (PubMed:10627524, PubMed:20416918, PubMed:26402374, PubMed:7882968). Required for the virus to move through the aphid (By similarity). The RTD domain of the protein is exposed on the surface of the particle and determines the vector specificity and intestinal tropism in the aphid (PubMed:7882968).[UniProtKB:P09516]<ref>PMID:10627524</ref> <ref>PMID:20416918</ref> <ref>PMID:26402374</ref> <ref>PMID:7882968</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP *) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP * contains the CP sequence fused with a structured domain, denoted (N)RTD, which is a key determinant of virus transmission. Though both CP and RTP * are involved in virus movement and aphid transmission, how RTP * is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP * does not impair the capsid structure, and the (N)RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP *s suggests a random insertion. | ||
| - | + | Structure of the turnip yellows virus particles.,Lai-Kee-Him J, Trapani S, Boissinot S, Reinbold C, Fallet C, Ancelin A, Lecorre F, Hoh F, Ziegler-Graff V, Brault V, Bron P Virology. 2025 Jun;607:110514. doi: 10.1016/j.virol.2025.110514. Epub 2025 Mar , 26. PMID:40179450<ref>PMID:40179450</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9q8j" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: Bron | + | <references/> |
| - | [[Category: Hoh | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Turnip yellows virus]] | ||
| + | [[Category: Brault V]] | ||
| + | [[Category: Bron P]] | ||
| + | [[Category: Hoh F]] | ||
| + | [[Category: Lai Kee Him J]] | ||
| + | [[Category: Trapani S]] | ||
Current revision
CryoEM structure of modified Turnip Yellows Virus devoid of minor capsid protein readthrough domain
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