9nn2
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Composite structure of HSV-1 helicase-primase in complex with a forked DNA and amenamevir== | |
| + | <StructureSection load='9nn2' size='340' side='right'caption='[[9nn2]], [[Resolution|resolution]] 3.10Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9nn2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1_strain_17 Human alphaherpesvirus 1 strain 17]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NN2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.1Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1BXD:~{N}-(2,6-dimethylphenyl)-~{N}-[2-[[4-(1,2,4-oxadiazol-3-yl)phenyl]amino]-2-oxidanylidene-ethyl]-1,1-bis(oxidanylidene)thiane-4-carboxamide'>A1BXD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nn2 OCA], [https://pdbe.org/9nn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nn2 RCSB], [https://www.ebi.ac.uk/pdbsum/9nn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nn2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HELI_HHV11 HELI_HHV11] Component of the helicase/primase complex. Unwinds the DNA at the replication forks and generates single-stranded DNA for both leading and lagging strand synthesis. The primase synthesizes short RNA primers on the lagging strand that the polymerase elongates using dNTPs. Possesses helicase-like motifs and therefore may act as the helicase subunit of the complex.[HAMAP-Rule:MF_04030] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Widespread herpesvirus infections are associated with various diseases. DNA replication of human herpes simplex virus type 1 (HSV-1) requires a helicase-primase (HP) complex of three core proteins: UL5, UL52, and UL8. This complex unwinds viral DNA and synthesizes primers for DNA replication, making it an attractive antiviral target. Although HP inhibitors pritelivir and amenamevir were identified through screening, their binding mechanisms remain unclear. Here, we report cryo-electron microscopy structures of HSV-1 HP bound to a forked DNA template alone and in complex with pritelivir or amenamevir. The structures reveal a bilobed architecture highlighting HP coordinated action at the replication fork and providing a structural basis for HP inhibition by illustrating precisely how pritelivir and amenamevir block helicase activity. Data lay a solid foundation for the development of improved antiviral therapies. | ||
| - | + | Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir.,Baranovskiy AG, He Q, Suwa Y, Morstadt LM, Babayeva ND, Lim CJ, Tahirov TH Sci Adv. 2025 Nov 7;11(45):eadz1989. doi: 10.1126/sciadv.adz1989. Epub 2025 Nov , 7. PMID:41202142<ref>PMID:41202142</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9nn2" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Human alphaherpesvirus 1 strain 17]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Babayeva ND]] | ||
| + | [[Category: Baranovskiy AG]] | ||
| + | [[Category: He Q]] | ||
| + | [[Category: Lim C]] | ||
| + | [[Category: Morstadt LM]] | ||
| + | [[Category: Tahirov TH]] | ||
Current revision
Composite structure of HSV-1 helicase-primase in complex with a forked DNA and amenamevir
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