9m84

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'''Unreleased structure'''
 
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The entry 9m84 is ON HOLD
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==Cryo-EM structure of Streptomyces coelicolor sigma factor shbA transcription initiation complex with shbA promoter==
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<StructureSection load='9m84' size='340' side='right'caption='[[9m84]], [[Resolution|resolution]] 3.61&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[9m84]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor_A3(2) Streptomyces coelicolor A3(2)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9M84 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9M84 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.61&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9m84 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9m84 OCA], [https://pdbe.org/9m84 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9m84 RCSB], [https://www.ebi.ac.uk/pdbsum/9m84 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9m84 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_STRCO RPOA_STRCO] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.[HAMAP-Rule:MF_00059]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In bacteria, principal sigma factors (sigma70 or sigmaA) transcribe housekeeping genes required for cell viability. Although most principal sigma genes are transcribed by the RNA polymerase (RNAP) holoenzyme containing the principal sigma factor itself, an extracytoplasmic function (ECF) sigma factor (sigmaShbA) governs transcription of the principal sigma factor gene (hrdB) in two model Streptomycetes. Here, we employed a combination of cryo-electron microscopy (cryo-EM) and bioinformatics to decipher how sigmaShbA-RNAP holoenzymes govern the transcription of hrdB genes in Streptomyces. A cryo-EM structure of Streptomyces coelicolor sigmaShbA-RNAP-promoter open (RPo) complex was solved at 2.97 A resolution. In combination with in vitro transcription assays, we demonstrate the unique structural features used by the sigmaShbA to recognize the hrdB promoter and form a transcription bubble. All Streptomyces genomes (603) tagged as 'reference' were retrieved from NCBI Datasets. The conserved protein sequences and genomic neighborhoods, as well as the promoter consensus sequences of sigmaShbA and sigmaHrdB homologs, support that the principal sigmaHrdB being governed by the ECF sigmaShbA is a common feature in Streptomyces. Overall, these results provide detailed molecular insights into the transcription of the principal sigmaHrdB gene and pave the way for globally modulating Streptomyces cell viability.
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Authors: Liu, G., Zheng, J.
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Molecular basis of Streptomyces ECF sigmaShbA factors transcribing principal sigmaHrdB genes.,Liu G, Yang X, Yan W, Wang Y, Yu F, Zheng J Nucleic Acids Res. 2025 Apr 22;53(8):gkaf339. doi: 10.1093/nar/gkaf339. PMID:40272361<ref>PMID:40272361</ref>
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Description: Cryo-EM structure of Streptomyces coelicolor sigma factor shbA transcription initiation complex with shbA promoter
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Liu, G]]
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<div class="pdbe-citations 9m84" style="background-color:#fffaf0;"></div>
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[[Category: Zheng, J]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Liu G]]
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[[Category: Zheng J]]

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Cryo-EM structure of Streptomyces coelicolor sigma factor shbA transcription initiation complex with shbA promoter

PDB ID 9m84

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