User:David Bogle/Sandbox 2

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== Scenes ==
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== Scenes == <StructureSection>
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<scene name='10/1076054/Iccg_disulfide_bridge/3'>Disulfide Bridge</scene>
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<scene name='10/1076054/Thermostability_wild_type/3'>D238 and S283</scene>
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<scene name='10/1076054/Iccg_disulfide_bridge/4'>Disulfide Bridge white</scene>
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<scene name='10/1076054/Iccg_disulfide_bridge/6'>Disulfide Bridge blue</scene>
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<scene name='10/1076054/Thermostability_wild_type/5'>D238 and S283</scene>
== Text ==
== Text ==
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<ref name="PET">{{Wikipedia|Polyethylene_terephthalate}}</ref>
 
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One of the most commonly used plastics is a polymer called Polyethylene Terephthalate, also known as [https://en.wikipedia.org/wiki/Polyethylene_terephthalate PET]<ref name="PET">{{Wikipedia|Polyethylene_terephthalate}}</ref>. PET is commonly used to make plastic bottles or food containers due to its thermal stability, transparency, and impermeability to liquids and gases. Unfortunately, these properties that make it desirable also make it non-biodegradable, meaning that we must recycle PET to avoid polluting the environment with it. Traditional methods, such as melting and reforming the plastic, destroy the mechanical properties of PET, eliminating the ability to recycle it and necessitating a new strategy for recycling PET.
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Main Article<ref name="main">PMID:32269349</ref>
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PET Recycling<ref name="recycle">Babaei, M., Jalilian, M., & Shahbaz, K. (2024). Chemical recycling of Polyethylene terephthalate: A mini-review. Journal of Environmental Chemical Engineering, 12(3), 112507. [https://doi.org/10.1016/j.jece.2024.112507. DOI: 10.1016/j.jece.2024.112507]</ref>
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LCC identification <ref name="LCC">PMID:22194294</ref>
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Engineering PET Hydrolases <ref name="Hydrolases">Jayasekara, S. K., Joni, H. D., Jayantha, B., Dissanayake, L., Mandrell, C., Sinharage, M. M. S., Molitor, R., Jayasekara, T., Sivakumar, P., & Jayakody, L. N. (2023). Trends in in-silico guided engineering of efficient polyethylene terephthalate (PET) hydrolyzing enzymes to enable bio-recycling and upcycling of PET. Computational and structural biotechnology journal, 21, 3513–3521. [https://doi.org/10.1016/j.csbj.2023.06.004​ DOI: 10.1016/j.csbj.2023.06.004]</ref>
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PET Mechanism <ref name="mechanism">Han, X., Liu, W., Huang, J. W., et al. (2017). Structural insight into catalytic mechanism of PET hydrolase. Nature Communications, 8, 2106. https://doi.org/10.1038/s41467-017-02255-z.Heredia-Guerrero, J. A., Heredia, A., García-Segura, R., & Benítez, J. J. (2009). Synthesis and characterization of a plant cutin mimetic polymer. Polymer, 50(24), 5633–5637. [https://doi.org/10.1016/j.polymer.2009.10.018​ DOI: 10.1016/j.polymer.2009.10.018]</ref>
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Biodegradation of PET <ref name="biodegradation">Hiraga, K., Taniguchi, I., Yoshida, S., Kimura, Y., & Oda, K. (2019). Biodegradation of waste PET: A sustainable solution for dealing with plastic pollution. EMBO Reports, 20(11), e49365. https://doi.org/10.15252/embr.201949365. [Published correction appears in EMBO Reports, 21(2), e49826. [https://doi.org/10.15252/embr.201949826.​ DOI: 10.15252/embr.201949826]</ref>
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Mechanism of PET Degradation <ref name="breakdown">PMID:29374183</ref>
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PET Degradation by TF <ref name="TF">PMID:24728714</ref>
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Dr.K <ref name="substrate">A binding model of the substrate 2-HE(MHET)3 in wild-type LLC (4eb0.pdb) was constructed and refined to mimic the 3D structure illustrated in Figure 2 of reference “1”. The software Maestro (Schrödinger, Inc; version 14.2.118) was used to construct the initial binding structure, followed by energy minimization in the context of the rigid protein that had previously been processed to add/refine all hydrogen atoms. The ligand model was then used without further modification to identify and illustrate the cited active-site residues.</ref>
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<ref group="xtra">PMID:15068885</ref>
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<references />
<references />
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===Additional Literature and Resources===
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<references group="xtra"/>
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====PDB Files====
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PDB Files
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Wild Type LCC: [https://www.rcsb.org/structure/4EB0 4EB0]
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Mutant ICCG: [https://www.rcsb.org/structure/6THT 6THT]

Current revision

== Scenes ==
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David Bogle

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