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1aqm

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ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS COMPLEXED WITH TRIS

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Retrieved from "http://52.214.119.220/wiki/index.php/1aqm"

Categories: Large Structures | Pseudoalteromonas haloplanktis | Aghajari N | Haser R

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-[[Image:1aqm.gif|left|200px]]<br />
-<applet load="1aqm" size="450" color="white" frame="true" align="right" spinBox="true"
-caption="1aqm, resolution 1.85&Aring;" />
-'''ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS COMPLEXED WITH TRIS'''<br />
-==Overview==
+==ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS COMPLEXED WITH TRIS==
-Alteromonas haloplanctis is a bacterium that flourishes in Antarctic, sea-water and it is considered as an extreme psychrophile. We have, determined the crystal structures of the alpha-amylase (AHA) secreted by, this bacterium, in its native state to 2.0 angstroms resolution as well as, in complex with Tris to 1.85 angstroms resolution. The structure of AHA, which is the first experimentally determined three-dimensional structure, of a psychrophilic enzyme, resembles those of other known alpha-amylases, of various origins with a surprisingly greatest similarity to mammalian, alpha-amylases. AHA contains a chloride ion which activates the hydrolytic, cleavage of substrate alpha-1,4-glycosidic bonds. The chloride binding, site is situated approximately 5 angstroms from the active site which ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9541387 (full description)]]
+<StructureSection load='1aqm' size='340' side='right'caption='[[1aqm]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1aqm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoalteromonas_haloplanktis Pseudoalteromonas haloplanktis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AQM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AQM FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aqm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aqm OCA], [https://pdbe.org/1aqm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aqm RCSB], [https://www.ebi.ac.uk/pdbsum/1aqm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aqm ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/AMY_PSEHA AMY_PSEHA]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aq/1aqm_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aqm ConSurf].
+<div style="clear:both"></div>
-==About this Structure==
+==See Also==
-AQM is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudoalteromonas_haloplanktis Pseudoalteromonas haloplanktis]] with CA, CL and TRS as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AQM OCA]].
+*[[Amylase 3D structures|Amylase 3D structures]]
+__TOC__
-==Reference==
+</StructureSection>
-Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor., Aghajari N, Feller G, Gerday C, Haser R, Protein Sci. 1998 Mar;7(3):564-72. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9541387 9541387]
+[[Category: Large Structures]]
 [[Category: Pseudoalteromonas haloplanktis]]
-[[Category: Single protein]]
+[[Category: Aghajari N]]
-[[Category: Aghajari, N.]]
+[[Category: Haser R]]
-[[Category: Haser, R.]]
-[[Category: CA]]
-[[Category: CL]]
-[[Category: TRS]]
-[[Category: 4-glucan-4-glucanohydrolase]]
-[[Category: alpha-1]]
-[[Category: alpha-amylase]]
-[[Category: beta-alpha-eight barrel]]
-[[Category: glycosidase inhibition]]
-[[Category: hydrolase]]
-[[Category: psychrophilic enzyme]]
-''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:40:39 2007''

1aqm

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ALPHA-AMYLASE FROM ALTEROMONAS HALOPLANCTIS COMPLEXED WITH TRIS

Structural highlights

Function

Evolutionary Conservation

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