Sandbox2QRU
From Proteopedia
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2QRU is known to be a hydrolase, most active on esterase. The structure is 276 amino acids long, which is approximately 30.14 kDa. Knowing 2QRU is an alpha/beta hydrolase, the literature helped give further information on possible experimental conditions and substrates that can be used to identify activity. However, experimental data has not been obtained yet. | 2QRU is known to be a hydrolase, most active on esterase. The structure is 276 amino acids long, which is approximately 30.14 kDa. Knowing 2QRU is an alpha/beta hydrolase, the literature helped give further information on possible experimental conditions and substrates that can be used to identify activity. However, experimental data has not been obtained yet. | ||
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== Structure/Sequence Analysis == | == Structure/Sequence Analysis == | ||
'''Sequence Analysis''' | '''Sequence Analysis''' | ||
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'''SPRITE''' | '''SPRITE''' | ||
| - | [[Image:2QRU(green)2LPR(yellow).png | thumb]]Analysis with Sprite displayed the proteins closest in structure to 2QRU. To do this, Sprite analyzed 2QRU for an active site with a motif of known catalytic function and compares to proteins known in the database. Among the proteins with the highest alignment (lowest RMSD) with a RMSD of 1.17 is 2LPR, a hydrolase. | + | [[Image:2QRU(green)2LPR(yellow).png | thumb]]Analysis with Sprite displayed the proteins closest in structure to 2QRU. To do this, Sprite analyzed 2QRU for an active site with a motif of known catalytic function and compares to proteins known in the database. Among the proteins with the highest alignment (lowest RMSD) with a RMSD of 1.17 is 2LPR, a hydrolase. The amino acids included in the active site was 102 Ser, 104 Gly, 219 Asp, 247 His. |
'''Chimera''' | '''Chimera''' | ||
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Investigation with InterPro showed the likely family of this enzyme was an alpha/beta hydrolase. Specifically, 2QRU was classified as a GDXG lipolytic enzyme. This family is generally associated with the hydrolysis of short fatty esters up to 8 carbons in length. Enzymes within this family are known to be involved in plant wall degradation and tryptophan synthesis. | Investigation with InterPro showed the likely family of this enzyme was an alpha/beta hydrolase. Specifically, 2QRU was classified as a GDXG lipolytic enzyme. This family is generally associated with the hydrolysis of short fatty esters up to 8 carbons in length. Enzymes within this family are known to be involved in plant wall degradation and tryptophan synthesis. | ||
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'''SwissDock''' | '''SwissDock''' | ||
Current revision
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