9rs2
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 9rs2 is ON HOLD until Paper Publication Authors: Li, H., Troycke, P., Yin, Z., Groll, M., Dickschat, J.S. Description: Sesterterpene Synthase from ...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Sesterterpene Synthase from Kitasatospora viridis in complex with the surrogate GFSPP== | |
| + | <StructureSection load='9rs2' size='340' side='right'caption='[[9rs2]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9rs2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora Kitasatospora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9RS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9RS2 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1JI6:[(2~{E},6~{E},10~{E},14~{E})-3,7,11,15,19-pentamethylicosa-2,6,10,14,18-pentaenyl]sulfanyl-phosphonooxy-phosphinic+acid'>A1JI6</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9rs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9rs2 OCA], [https://pdbe.org/9rs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9rs2 RCSB], [https://www.ebi.ac.uk/pdbsum/9rs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9rs2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A561SAF2_9ACTN A0A561SAF2_9ACTN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Terpene synthases produce a remarkable structural diversity from acyclic precursors through complex carbocation cascades. Here, we report the crystal structure of the bacterial sesterterpene synthase StvirS bound to geranylfarnesyl thiopyrophosphate (GFSPP), revealing a preorganized active site that enforces a defined folding of the C25 backbone. Guided by this structure, active-site engineering at 11 positions yielded 23 enzyme variants and 13 new sesterterpenes. Specific substitutions altered reaction trajectories, stereochemistry, or induced premature termination, emphasizing the sensitivity of StvirS to subtle structural changes. Isotopic labeling established the absolute configurations of 11 products and experimentally confirmed a conserved cyclization pathway. These results illustrate how precise noncovalent interactions govern terpene biosynthesis and highlight the promise of structure-based design to reprogram terpene cyclase reactivity. | ||
| - | + | Structure-Guided Engineering of a Bacterial Sesterterpene Synthase for Sesterviridene Diversification.,Li H, Troycke P, Yin Z, Groll M, Dickschat JS J Am Chem Soc. 2025 Sep 11. doi: 10.1021/jacs.5c11309. PMID:40931719<ref>PMID:40931719</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 9rs2" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: Troycke | + | __TOC__ |
| - | [[Category: Yin | + | </StructureSection> |
| + | [[Category: Kitasatospora]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Dickschat JS]] | ||
| + | [[Category: Groll M]] | ||
| + | [[Category: Li H]] | ||
| + | [[Category: Troycke P]] | ||
| + | [[Category: Yin Z]] | ||
Current revision
Sesterterpene Synthase from Kitasatospora viridis in complex with the surrogate GFSPP
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Categories: Kitasatospora | Large Structures | Dickschat JS | Groll M | Li H | Troycke P | Yin Z
