1wa8

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[[Image:1wa8.gif|left|200px]]
 
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==Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria==
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The line below this paragraph, containing "STRUCTURE_1wa8", creates the "Structure Box" on the page.
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<StructureSection load='1wa8' size='340' side='right'caption='[[1wa8]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wa8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv] and [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_variant_bovis Mycobacterium tuberculosis variant bovis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WA8 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wa8 OCA], [https://pdbe.org/1wa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wa8 RCSB], [https://www.ebi.ac.uk/pdbsum/1wa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wa8 ProSAT]</span></td></tr>
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{{STRUCTURE_1wa8| PDB=1wa8 | SCENE= }}
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</table>
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== Function ==
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'''SOLUTION STRUCTURE OF THE CFP-10.ESAT-6 COMPLEX. MAJOR VIRULENCE DETERMINANTS OF PATHOGENIC MYCOBACTERIA'''
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[https://www.uniprot.org/uniprot/ESXB_MYCBO ESXB_MYCBO] A secreted protein. Acts as a strong host T-cell antigen. Involved in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. Might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA.[UniProtKB:P9WNK5]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wa8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wa8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.
The secreted Mycobacterium tuberculosis complex proteins CFP-10 and ESAT-6 have recently been shown to play an essential role in tuberculosis pathogenesis. We have determined the solution structure of the tight, 1:1 complex formed by CFP-10 and ESAT-6, and employed fluorescence microscopy to demonstrate specific binding of the complex to the surface of macrophage and monocyte cells. A striking feature of the complex is the long flexible arm formed by the C-terminus of CFP-10, which was found to be essential for binding to the surface of cells. The surface features of the CFP-10.ESAT-6 complex, together with observed binding to specific host cells, strongly suggest a key signalling role for the complex, in which binding to cell surface receptors leads to modulation of host cell behaviour to the advantage of the pathogen.
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==About this Structure==
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Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6.,Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:15973432<ref>PMID:15973432</ref>
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1WA8 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_bovis Mycobacterium bovis] and [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6., Renshaw PS, Lightbody KL, Veverka V, Muskett FW, Kelly G, Frenkiel TA, Gordon SV, Hewinson RG, Burke B, Norman J, Williamson RA, Carr MD, EMBO J. 2005 Jul 20;24(14):2491-8. Epub 2005 Jun 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15973432 15973432]
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</div>
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[[Category: Mycobacterium bovis]]
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<div class="pdbe-citations 1wa8" style="background-color:#fffaf0;"></div>
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[[Category: Mycobacterium tuberculosis]]
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== References ==
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[[Category: Protein complex]]
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<references/>
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[[Category: Burke, B.]]
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__TOC__
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[[Category: Carr, M D.]]
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</StructureSection>
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[[Category: Frenkiel, T A.]]
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[[Category: Large Structures]]
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[[Category: Gordon, S V.]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Hewinson, R G.]]
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[[Category: Mycobacterium tuberculosis variant bovis]]
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[[Category: Kelly, G.]]
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[[Category: Burke B]]
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[[Category: Lightbody, K L.]]
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[[Category: Carr MD]]
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[[Category: Muskett, F W.]]
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[[Category: Frenkiel TA]]
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[[Category: Norman, J.]]
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[[Category: Gordon SV]]
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[[Category: Renshaw, P S.]]
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[[Category: Hewinson RG]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: Kelly G]]
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[[Category: Veverka, V.]]
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[[Category: Lightbody KL]]
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[[Category: Williamson, R A.]]
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[[Category: Muskett FW]]
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[[Category: Protein structure initiative]]
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[[Category: Norman J]]
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[[Category: Psi]]
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[[Category: Renshaw PS]]
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[[Category: Psi,protein structure initiative,tb structural genomics consortium]]
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[[Category: Veverka V]]
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[[Category: Tbsgc]]
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[[Category: Williamson RA]]
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[[Category: Tuberculosis,cfp-10,esat-6,helix-turn-helix,four helix bundle,mycobacteria,pathogenesis,nmr,solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:22:34 2008''
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Current revision

Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

PDB ID 1wa8

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