1wlf

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[[Image:1wlf.jpg|left|200px]]
 
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==Structure of the N-terminal domain of PEX1 AAA-ATPase: Characterization of a putative adaptor-binding domain==
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The line below this paragraph, containing "STRUCTURE_1wlf", creates the "Structure Box" on the page.
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<StructureSection load='1wlf' size='340' side='right'caption='[[1wlf]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1wlf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WLF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1wlf| PDB=1wlf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wlf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wlf OCA], [https://pdbe.org/1wlf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wlf RCSB], [https://www.ebi.ac.uk/pdbsum/1wlf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wlf ProSAT]</span></td></tr>
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</table>
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'''Structure of the N-terminal domain of PEX1 AAA-ATPase: Characterization of a putative adaptor-binding domain'''
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== Function ==
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[https://www.uniprot.org/uniprot/PEX1_MOUSE PEX1_MOUSE] Required for stability of PEX5 and protein import into the peroxisome matrix. Anchored by PEX26 to peroxisome membranes, possibly to form heteromeric AAA ATPase complexes required for the import of proteins into peroxisomes (By similarity).
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Peroxisomes are responsible for several pathways in primary metabolism, including beta-oxidation and lipid biosynthesis. PEX1 and PEX6 are hexameric AAA-type ATPases, both of which are indispensable in targeting over 50 peroxisomal resident proteins from the cytosol to the peroxisomes. Although the tandem AAA-ATPase domains in the central region of PEX1 and PEX6 are highly similar, the N-terminal sequences are unique. To better understand the distinct molecular function of these two proteins, we analyzed the unique N-terminal domain (NTD) of PEX1. Extensive computational analysis revealed weak similarity (&lt;10% identity) of PEX1 NTD to the N-terminal domains of other membrane-related type II AAA-ATPases, such as VCP (p97) and NSF. We have determined the crystal structure of mouse PEX1 NTD at 2.05-A resolution, which clearly demonstrated that the domain belongs to the double-psi-barrel fold family found in the other AAA-ATPases. The N-domains of both VCP and NSF are structural neighbors of PEX1 NTD with a 2.7- and 2.1-A root mean square deviation of backbone atoms, respectively. Our findings suggest that the supradomain architecture, which is composed of a single N-terminal domain followed by tandem AAA domains, is a common feature of organellar membrane-associating AAA-ATPases. We propose that PEX1 functions as a protein unfoldase in peroxisomal biogenesis, using its N-terminal putative adaptor-binding domain.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/1wlf_consurf.spt"</scriptWhenChecked>
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WLF OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain., Shiozawa K, Maita N, Tomii K, Seto A, Goda N, Akiyama Y, Shimizu T, Shirakawa M, Hiroaki H, J Biol Chem. 2004 Nov 26;279(48):50060-8. Epub 2004 Aug 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15328346 15328346]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wlf ConSurf].
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[[Category: Akiyama, Y.]]
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<div style="clear:both"></div>
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[[Category: Goda, N.]]
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__TOC__
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[[Category: Hiroaki, H.]]
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</StructureSection>
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[[Category: Maita, N.]]
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[[Category: Large Structures]]
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[[Category: Seto, A.]]
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[[Category: Mus musculus]]
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[[Category: Shimizu, T.]]
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[[Category: Akiyama Y]]
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[[Category: Shiozawa, K.]]
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[[Category: Goda N]]
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[[Category: Shirakawa, M.]]
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[[Category: Hiroaki H]]
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[[Category: Tochio, H.]]
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[[Category: Maita N]]
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[[Category: Tomii, K.]]
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[[Category: Seto A]]
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[[Category: N-terminal domain]]
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[[Category: Shimizu T]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:50:17 2008''
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[[Category: Shiozawa K]]
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[[Category: Shirakawa M]]
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[[Category: Tochio H]]
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[[Category: Tomii K]]

Current revision

Structure of the N-terminal domain of PEX1 AAA-ATPase: Characterization of a putative adaptor-binding domain

PDB ID 1wlf

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