2a3r

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="2a3r" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a3r, resolution 2.60&Aring;" /> '''Crystal Structure o...)
Current revision (08:16, 25 October 2023) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2a3r.gif|left|200px]]<br />
 
-
<applet load="2a3r" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2a3r, resolution 2.60&Aring;" />
 
-
'''Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate'''<br />
 
-
==Overview==
+
==Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate==
-
The human sulfotransferase, SULT1A3, catalyzes specifically the, sulfonation of monoamines such as dopamine, epinephrine, and, norepinephrine. SULT1A3 also has a unique 3,4-dihydroxyphenylalanine, (Dopa)/tyrosine-sulfating activity that is preferentially toward their, D-form enantiomers and can be stimulated dramatically by Mn2+. To further, our understanding of the molecular basis for the unique substrate, specificity of this enzyme, we solved the crystal structure of human, SULT1A3, complexed with dopamine and 3'-phosphoadenosine 5'-phosphate, at, 2.6 A resolution and carried out autodocking analysis with D-Dopa. The, structure of SULT1A3 enzyme-ligand complex clearly showed that residue, Glu146 can form electrostatic interaction with dopamine and may play a, pivotal role in the stereoselectivity and sulfating activity. On the other, hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the, Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting, role in the stereoselectivity and sulfating activity.
+
<StructureSection load='2a3r' size='340' side='right'caption='[[2a3r]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2a3r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A3R FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3P:ADENOSINE-3-5-DIPHOSPHATE'>A3P</scene>, <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a3r OCA], [https://pdbe.org/2a3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a3r RCSB], [https://www.ebi.ac.uk/pdbsum/2a3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a3r ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/ST1A3_HUMAN ST1A3_HUMAN]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a3/2a3r_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a3r ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The human sulfotransferase, SULT1A3, catalyzes specifically the sulfonation of monoamines such as dopamine, epinephrine, and norepinephrine. SULT1A3 also has a unique 3,4-dihydroxyphenylalanine (Dopa)/tyrosine-sulfating activity that is preferentially toward their D-form enantiomers and can be stimulated dramatically by Mn2+. To further our understanding of the molecular basis for the unique substrate specificity of this enzyme, we solved the crystal structure of human SULT1A3, complexed with dopamine and 3'-phosphoadenosine 5'-phosphate, at 2.6 A resolution and carried out autodocking analysis with D-Dopa. The structure of SULT1A3 enzyme-ligand complex clearly showed that residue Glu146 can form electrostatic interaction with dopamine and may play a pivotal role in the stereoselectivity and sulfating activity. On the other hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting role in the stereoselectivity and sulfating activity.
-
==Disease==
+
Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate.,Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:16083857<ref>PMID:16083857</ref>
-
Known diseases associated with this structure: Alzheimer disease-4 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600759 600759]], Cardiomyopathy, dilated, 1V OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=600759 600759]]
+
-
==About this Structure==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
2A3R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with A3P and LDP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A3R OCA].
+
</div>
 +
<div class="pdbe-citations 2a3r" style="background-color:#fffaf0;"></div>
-
==Reference==
+
==See Also==
-
Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate., Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16083857 16083857]
+
*[[Sulfotransferase 3D structures|Sulfotransferase 3D structures]]
-
[[Category: Aryl sulfotransferase]]
+
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: An, X.M.]]
+
[[Category: An XM]]
-
[[Category: Chang, W.R.]]
+
[[Category: Chang WR]]
-
[[Category: Li, H.T.]]
+
[[Category: Li HT]]
-
[[Category: Li, M.]]
+
[[Category: Li M]]
-
[[Category: Liu, M.C.]]
+
[[Category: Liu MC]]
-
[[Category: Lu, J.H.]]
+
[[Category: Lu JH]]
-
[[Category: Zhang, J.P.]]
+
[[Category: Zhang JP]]
-
[[Category: A3P]]
+
-
[[Category: LDP]]
+
-
[[Category: complex]]
+
-
[[Category: crystal structure]]
+
-
[[Category: dopamine]]
+
-
[[Category: sulfotransferase]]
+
-
[[Category: sult1a3]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:45:50 2007''
+

Current revision

Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate

PDB ID 2a3r

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a3r"
Personal tools