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2a5g

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(New page: 200px<br /> <applet load="2a5g" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a5g, resolution 2.66&Aring;" /> '''Cholera toxin A1 su...)
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[[Image:2a5g.gif|left|200px]]<br />
 
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<applet load="2a5g" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2a5g, resolution 2.66&Aring;" />
 
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'''Cholera toxin A1 subunit bound to ARF6(Q67L)'''<br />
 
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==Overview==
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==Cholera toxin A1 subunit bound to ARF6(Q67L)==
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The Vibrio cholerae bacterium causes devastating diarrhea when it infects, the human intestine. The key event is adenosine diphosphate, (ADP)-ribosylation of the human signaling protein GSalpha, catalyzed by, the cholera toxin A1 subunit (CTA1). This reaction is allosterically, activated by human ADP-ribosylation factors (ARFs), a family of essential, and ubiquitous G proteins. Crystal structures of a CTA1:ARF6-GTP, (guanosine triphosphate) complex reveal that binding of the human, activator elicits dramatic changes in CTA1 loop regions that allow, nicotinamide adenine dinucleotide (NAD+) to bind to the active site. The, extensive toxin:ARF-GTP interface surface mimics ARF-GTP recognition of, normal cellular protein partners, which suggests that the toxin has, evolved to exploit promiscuous binding properties of ARFs.
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<StructureSection load='2a5g' size='340' side='right'caption='[[2a5g]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2a5g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A5G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a5g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5g OCA], [https://pdbe.org/2a5g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a5g RCSB], [https://www.ebi.ac.uk/pdbsum/2a5g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a5g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARF6_HUMAN ARF6_HUMAN] GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.<ref>PMID:7589240</ref> <ref>PMID:14978216</ref> <ref>PMID:11266366</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/2a5g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2a5g ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2A5G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with MG, NA and GTP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/NAD(+)--diphthamide_ADP-ribosyltransferase NAD(+)--diphthamide ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.36 2.4.2.36] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2A5G OCA].
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*[[Cholera toxin 3D structures|Cholera toxin 3D structures]]
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*[[User:David Solfiell/sandbox 1|User:David Solfiell/sandbox 1]]
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==Reference==
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== References ==
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Structural basis for the activation of cholera toxin by human ARF6-GTP., O'Neal CJ, Jobling MG, Holmes RK, Hol WG, Science. 2005 Aug 12;309(5737):1093-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16099990 16099990]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: NAD(+)--diphthamide ADP-ribosyltransferase]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Hol, W.G.J.]]
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[[Category: Hol WGJ]]
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[[Category: Holmes, R.K.]]
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[[Category: Holmes RK]]
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[[Category: Jobling, M.G.]]
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[[Category: Jobling MG]]
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[[Category: Neal, C.J.O.]]
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[[Category: O'Neal CJ]]
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[[Category: GTP]]
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[[Category: MG]]
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[[Category: NA]]
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[[Category: protein transport/transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 20:46:35 2007''
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Current revision

Cholera toxin A1 subunit bound to ARF6(Q67L)

PDB ID 2a5g

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