1x77

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[[Image:1x77.gif|left|200px]]
 
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==Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN==
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The line below this paragraph, containing "STRUCTURE_1x77", creates the "Structure Box" on the page.
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<StructureSection load='1x77' size='340' side='right'caption='[[1x77]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1x77]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X77 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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{{STRUCTURE_1x77| PDB=1x77 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x77 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x77 OCA], [https://pdbe.org/1x77 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x77 RCSB], [https://www.ebi.ac.uk/pdbsum/1x77 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x77 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1x77 TOPSAN]</span></td></tr>
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</table>
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'''Crystal struture of a NAD(P)H-dependent FMN reductase complexed with FMN'''
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== Function ==
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[https://www.uniprot.org/uniprot/FMNRE_PSEAE FMNRE_PSEAE] Has NAD(P)H-dependent FMN reductase activity.<ref>PMID:16552139</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x77_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x77 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.
The availability of high-intensity synchrotron facilities, technological advances in data-collection techniques and improved data-reduction and crystallographic software have ushered in a new era in high-throughput macromolecular crystallography. Here, the de novo automated crystal structure determination at 1.28 A resolution of an NAD(P)H-dependent FMN reductase flavoprotein from Pseudomonas aeruginosa PA01-derived protein Q9I4D4 using the anomalous signal from an unusually small number of S atoms is reported. Although this protein lacks the flavodoxin key fingerprint motif [(T/S)XTGXT], it has been confirmed to bind flavin mononucleotide and the binding site was identified via X-ray crystallography. This protein contains a novel flavin mononucleotide-binding site GSLRSGSYN, which has not been previously reported. Detailed statistics pertaining to sulfur phasing and other factors contributing to structure determination are discussed. Structural comparisons of the apoenzyme and the protein complexed with flavin mononucleotide show conformational changes on cofactor binding. NADPH-dependent activity has been confirmed with biochemical assays.
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==About this Structure==
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Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal.,Agarwal R, Bonanno JB, Burley SK, Swaminathan S Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:16552139<ref>PMID:16552139</ref>
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1X77 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_pao1 Pseudomonas aeruginosa pao1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X77 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure determination of an FMN reductase from Pseudomonas aeruginosa PA01 using sulfur anomalous signal., Agarwal R, Bonanno JB, Burley SK, Swaminathan S, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):383-91. Epub 2006, Mar 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16552139 16552139]
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</div>
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[[Category: Pseudomonas aeruginosa pao1]]
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<div class="pdbe-citations 1x77" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Agarwal, R.]]
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<references/>
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[[Category: Burley, S K.]]
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__TOC__
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[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
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</StructureSection>
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[[Category: Swaminathan, S.]]
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[[Category: Large Structures]]
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[[Category: Fmn bound]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Fmn reductase]]
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[[Category: Agarwal R]]
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[[Category: New york structural genomix research consortium]]
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[[Category: Burley SK]]
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[[Category: Nysgxrc]]
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[[Category: Swaminathan S]]
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[[Category: Structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:39:11 2008''
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Current revision

Crystal structure of a NAD(P)H-dependent FMN reductase complexed with FMN

PDB ID 1x77

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