1xdp

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[[Image:1xdp.gif|left|200px]]
 
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==Crystal Structure of the E.coli Polyphosphate Kinase in complex with AMPPNP==
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The line below this paragraph, containing "STRUCTURE_1xdp", creates the "Structure Box" on the page.
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<StructureSection load='1xdp' size='340' side='right'caption='[[1xdp]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xdp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XDP FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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{{STRUCTURE_1xdp| PDB=1xdp | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xdp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xdp OCA], [https://pdbe.org/1xdp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xdp RCSB], [https://www.ebi.ac.uk/pdbsum/1xdp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xdp ProSAT]</span></td></tr>
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</table>
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'''Crystal Structure of the E.coli Polyphosphate Kinase in complex with AMPPNP'''
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== Function ==
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[https://www.uniprot.org/uniprot/PPK1_ECOLI PPK1_ECOLI] Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). Can form linear polymers of orthophosphate with chain lengths up to 1000 or more. Can use GTP instead of ATP, but the efficiency of GTP is 5% that of ATP. Also exhibits several other enzymatic activities, which include: ATP synthesis from polyP in the presence of excess ADP, general nucleoside-diphosphate kinase activity, linear guanosine 5'-tetraphosphate (ppppG) synthesis and autophosphorylation.<ref>PMID:10660553</ref> <ref>PMID:8962061</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Polyphosphate (polyP), a linear polymer of hundreds of orthophosphate residues, exists in all tested cells in nature, from pathogenic bacteria to mammals. In bacteria, polyP has a crucial role in stress responses and stationary-phase survival. Polyphosphate kinase (PPK) is the principal enzyme that catalyses the synthesis of polyP in bacteria. It has been shown that PPK is required for bacterial motility, biofilm formation and the production of virulence factors. PPK inhibitors may thus provide a unique therapeutic opportunity against antibiotic-resistant pathogens. Here, we report crystal structures of full-length Escherichia coli PPK and its complex with AMPPNP (beta-gamma-imidoadenosine 5-phosphate). PPK forms an interlocked dimer, with each 80 kDa monomer containing four structural domains. The PPK active site is located in a tunnel, which contains a unique ATP-binding pocket and may accommodate the translocation of synthesized polyP. The PPK structure has laid the foundation for understanding the initiation of polyP synthesis by PPK.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xd/1xdp_consurf.spt"</scriptWhenChecked>
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1XDP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XDP OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis., Zhu Y, Huang W, Lee SS, Xu W, EMBO Rep. 2005 Jul;6(7):681-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15947782 15947782]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xdp ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Polyphosphate kinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Huang W]]
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[[Category: Huang, W.]]
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[[Category: Lee SS]]
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[[Category: Lee, S S.]]
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[[Category: Xu W]]
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[[Category: Xu, W.]]
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[[Category: Zhu Y]]
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[[Category: Zhu, Y.]]
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[[Category: Amppnp]]
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[[Category: E coli polyphosphate kinase]]
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[[Category: Ppk]]
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[[Category: Ppk complex with amppnp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:53:56 2008''
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Current revision

Crystal Structure of the E.coli Polyphosphate Kinase in complex with AMPPNP

PDB ID 1xdp

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