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1xjx

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[[Image:1xjx.gif|left|200px]]
 
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==The crystal structures of the DNA binding sites of the RUNX1 transcription factor==
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The line below this paragraph, containing "STRUCTURE_1xjx", creates the "Structure Box" on the page.
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<StructureSection load='1xjx' size='340' side='right'caption='[[1xjx]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1xjx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XJX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xjx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjx OCA], [https://pdbe.org/1xjx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xjx RCSB], [https://www.ebi.ac.uk/pdbsum/1xjx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xjx ProSAT]</span></td></tr>
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{{STRUCTURE_1xjx| PDB=1xjx | SCENE= }}
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</table>
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__TOC__
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'''The crystal structures of the DNA binding sites of the RUNX1 transcription factor'''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Kitayner M]]
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==Overview==
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[[Category: Rabinovich D]]
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Runt-domain (RD) proteins are transcription factors that play fundamental roles in various developmental pathways. They bind specifically to DNA sequences of the general form PyGPyGGTPy (Py = pyrimidine), through which they regulate transcription of target genes. The DNA duplex TCTGCGGTC/TGACCGCAG, incorporating the binding site for the RD transcription factors (bold), was crystallized in space group P4(3). X-ray analysis of two crystals diffracting to 1.7 and 2.0 angstroms resolution, which had slight variations in their unit-cell parameters, revealed two distinct conformations of the A-DNA helix. The two crystal structures possessed several structure and hydration features that had previously been observed in A-DNA duplexes. A comparative analysis of the present A-DNA structures and those of previously reported B-DNA crystal structures of RD-binding sites in free and protein-bound states showed the various duplexes to display several common features. Within this series, the present A-DNA duplexes adopt two conformations along the pathway from the canonical A-DNA to the B-DNA forms and the protein-bound helices display conformational features that are intermediate between those of the current A-DNA structures and that of the B-DNA-type helix of the free RD target. Based on these data and energy considerations, it is likely that the propensity of the RD-binding site to adopt the A-DNA or B-DNA conformation in solution depends on the sequence context and environmental conditions, and that the transition from either DNA form to the protein-bound conformation involves a small energy barrier.
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[[Category: Rozenberg H]]
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[[Category: Shakked Z]]
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==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJX OCA].
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==Reference==
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Structures of the DNA-binding site of Runt-domain transcription regulators., Kitayner M, Rozenberg H, Rabinovich D, Shakked Z, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):236-46. Epub 2005, Feb 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15735333 15735333]
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[[Category: Kitayner, M.]]
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[[Category: Rabinovich, D.]]
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[[Category: Rozenberg, H.]]
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[[Category: Shakked, Z.]]
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[[Category: A-dna]]
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[[Category: Double helix]]
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[[Category: Overhanging base]]
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[[Category: Runx1]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:07:33 2008''
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Current revision

The crystal structures of the DNA binding sites of the RUNX1 transcription factor

PDB ID 1xjx

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