1xnz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:51, 14 February 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1xnz.jpg|left|200px]]
 
-
<!--
+
==Crystal Structure of Mn(II) form of E. coli. Methionine Aminopeptidase in complex with 5-(2-chlorophenyl)furan-2-carboxylic acid==
-
The line below this paragraph, containing "STRUCTURE_1xnz", creates the "Structure Box" on the page.
+
<StructureSection load='1xnz' size='340' side='right'caption='[[1xnz]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1xnz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XNZ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.52&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FCD:5-(2-CHLOROPHENYL)FURAN-2-CARBOXYLIC+ACID'>FCD</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
{{STRUCTURE_1xnz| PDB=1xnz | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xnz OCA], [https://pdbe.org/1xnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xnz RCSB], [https://www.ebi.ac.uk/pdbsum/1xnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xnz ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MAP1_ECOLI MAP1_ECOLI] Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.[HAMAP-Rule:MF_01974]<ref>PMID:20521764</ref> <ref>PMID:3027045</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xn/1xnz_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xnz ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal Structure of Mn(II) form of E. coli. Methionine Aminopeptidase in complex with 5-(2-chlorophenyl)furan-2-carboxylic acid'''
+
==See Also==
-
 
+
*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
-
 
+
== References ==
-
==Overview==
+
<references/>
-
Methionine aminopeptidase (MetAP) enzymes require a divalent metal ion such as Mn(II), Fe(II), Co(II), Ni(II), or Zn(II) for its removal of the N-terminal methionine from newly synthesized proteins, but it is not certain which of these ions is most important in vivo. Metalloform-selective MetAP inhibitors could be valuable for defining which metals are physiologically relevant for MetAP activation and could serve as leads for development of new therapeutic agents. We have screened a library of 43 736 small drug-like molecules against Escherichia coli MetAP and identified two groups of potent and highly metalloform-selective inhibitors of the Co(II)-form, and of the Mn(II)-form, of this enzyme. Compound 1 is 790-fold more selective for the Co(II)-form, while compound 4 is over 640-fold more potent toward the Mn(II)-form. The X-ray structure of a di-Mn(II) form of E. coli MetAP complexed with the Mn(II)-form-selective compound 4 was obtained, and it shows that the inhibitor interacts with both Mn(II) ions through the two oxygen atoms of its free carboxylate group. The preferential coordination of the hard (oxygen) donors to Mn(II) may contribute to its superb selectivity toward the Mn(II)-form.
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1XNZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XNZ OCA].
+
-
 
+
-
==Reference==
+
-
Metalloform-selective inhibitors of escherichia coli methionine aminopeptidase and X-ray structure of a Mn(II)-form enzyme complexed with an inhibitor., Ye QZ, Xie SX, Huang M, Huang WJ, Lu JP, Ma ZQ, J Am Chem Soc. 2004 Nov 3;126(43):13940-1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15506752 15506752]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Methionyl aminopeptidase]]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Huang M]]
-
[[Category: Huang, M.]]
+
[[Category: Huang W-J]]
-
[[Category: Huang, W J.]]
+
[[Category: Lu J-P]]
-
[[Category: Lu, J P.]]
+
[[Category: Ma Z-Q]]
-
[[Category: Ma, Z Q.]]
+
[[Category: Xie S-X]]
-
[[Category: Xie, S X.]]
+
[[Category: Ye Q-Z]]
-
[[Category: Ye, Q Z.]]
+
-
[[Category: Complex]]
+
-
[[Category: Inhibitor]]
+
-
[[Category: Methionine aminopeptidase]]
+
-
[[Category: Structure]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:16:51 2008''
+

Current revision

Crystal Structure of Mn(II) form of E. coli. Methionine Aminopeptidase in complex with 5-(2-chlorophenyl)furan-2-carboxylic acid

PDB ID 1xnz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xnz"
Personal tools